Cellular and molecular drivers of differential organ growth: insights from the limbs of Monodelphis domestica

Anna Dowling, Carolyn Doroba, Jennifer A. Maier, Lorna Cohen, John VandeBerg, Karen E. Sears

Research output: Contribution to journalArticle

5 Scopus citations

Abstract

A fundamental question in biology is “how is growth differentially regulated during development to produce organs of particular sizes?” We used a new model system for the study of differential organ growth, the limbs of the opossum (Monodelphis domestica), to investigate the cellular and molecular basis of differential organ growth in mammals. Opossum forelimbs grow much faster than hindlimbs, making opossum limbs an exceptional system with which to study differential growth. We first used the great differences in opossum forelimb and hindlimb growth to identify cellular processes and molecular signals that underlie differential limb growth. We then used organ culture and pharmacological addition of FGF ligands and inhibitors to test the role of the Fgf/Mitogen-activated protein kinases (MAPK) signaling pathway in driving these cellular processes. We found that molecular signals from within the limb drive differences in cell proliferation that contribute to the differential growth of the forelimb and hindlimbs of opossums. We also found that alterations in the Fgf/MAPK pathway can generate differences in cell proliferation that mirror those observed between wild-type forelimb and hindlimbs of opossums and that manipulation of Fgf/MAPK signaling affects downstream focal adhesion-extracellular matrix (FA-ECM) and Wnt signaling in opossum limbs. Taken together, these findings suggest that evolutionary changes in the Fgf/MAPK pathway could help drive the observed differences in cell behaviors and growth in opossum forelimb and hindlimbs.

Original languageEnglish (US)
Pages (from-to)1-9
Number of pages9
JournalDevelopment Genes and Evolution
DOIs
StateAccepted/In press - May 18 2016
Externally publishedYes

Keywords

  • Cell death
  • Cell proliferation
  • FA-ECM signaling
  • Fgf signaling
  • Opossum
  • Wnt signaling

ASJC Scopus subject areas

  • Developmental Biology
  • Genetics

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