Cell volume changes in relation to the cell cycle of differentiating erythroleukemic cells

Yair Gazitt, Arline D. Deitch, Paul A. Marks, Richard A. Rifkind

Research output: Contribution to journalArticlepeer-review

30 Scopus citations


Murine erythroleukemic cells (MELC) were synchronized by sequential exposure to thymidine and hydroxyurea. Upon removal from hydroxyurea, cells cultured with or without agents that induce erythroid differentiation, such as hexamethylene bisacetamide (HMBA) or dimethylsulfoxide (Me2SO), proceed through S, G2 and mitosis with the same kinetics: S phase averages 5 h and G2 plus mitosis, 2 h. Cells cultured with HMBA and Me2SO remain in the subsequent G1 for 5-7 h, compared with an average of only 3 h for cells cultured without inducer. Modal cell volume doubles as the cells proceed from G1 to G2. During the inducer-mediated prolonged G1, MELC retain a small cell volume. In cultures of non-synchronous MELC, inducers also increase the G1 fraction, as well as the proportion of small cells. An Me2SO-resistant MELC variant (DR10), cultured with Me2SO, shows little prolongation of G1 and little difference in the modal cell volume compared with cells without inducer. However, HMBA, which induces differentiation of DR10 cells, prolongs G1 and increases the proportion of small cells. These studies indicate that early changes in cell volume associated with induction of MELC to differentiate, in large part reflect alterations in the cell cycle. Evidence is presented which suggests that only one round of DNA synthesis in the presence of inducer may be necessary to initiate differentiation.

Original languageEnglish (US)
Pages (from-to)413-420
Number of pages8
JournalExperimental Cell Research
Issue number2
StatePublished - Dec 1978
Externally publishedYes

ASJC Scopus subject areas

  • Cell Biology


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