Cell density is a critical determinant of aromatase expression in adipose stromal cells

Sagar Ghosh, Yanfen Hu, Rong Li

Research output: Contribution to journalArticle

6 Citations (Scopus)

Abstract

Obesity is associated with an increased risk of breast cancer among postmenopausal women. This is at least partly due to excessive estrogen production in adipose tissue of obese women. Aromatase, the key enzyme in estrogen biosynthesis, is an important target in endocrine therapy for estrogen receptor (ER)-positive postmenopausal breast cancer. In this study we show that high confluency of human adipose stromal cells (ASCs) cultured in vitro can significantly stimulate aromatase gene expression and reduce the expression of breast tumor suppressor BRCA1 and members of the NR4A orphan nuclear family. Furthermore, small interfering RNA (siRNA)-mediated knockdown of Nurr1, a member of the NR4A family, substantially increased aromatase expression. Lastly,wefound that the cell density-triggered inducibility of aromatase expression varies in ASCs isolated from different disease-free individuals. Our finding highlights the impact of increased cell number on estrogen biosynthesis as in the case of excessive adiposity.

Original languageEnglish (US)
Pages (from-to)231-236
Number of pages6
JournalJournal of Steroid Biochemistry and Molecular Biology
Volume118
Issue number4-5
DOIs
StatePublished - Feb 28 2010

Fingerprint

Aromatase
Stromal Cells
Cell Count
Estrogens
Biosynthesis
Breast Neoplasms
Adiposity
Nuclear Family
Gene expression
Estrogen Receptors
Small Interfering RNA
Adipose Tissue
Tumors
Obesity
Tissue
Gene Expression
Enzymes
Therapeutics

Keywords

  • Adipose stromal cells
  • Aromatase
  • Cell density
  • NR4A

ASJC Scopus subject areas

  • Molecular Medicine
  • Endocrinology, Diabetes and Metabolism
  • Molecular Biology
  • Cell Biology
  • Endocrinology
  • Clinical Biochemistry
  • Biochemistry
  • Medicine(all)

Cite this

Cell density is a critical determinant of aromatase expression in adipose stromal cells. / Ghosh, Sagar; Hu, Yanfen; Li, Rong.

In: Journal of Steroid Biochemistry and Molecular Biology, Vol. 118, No. 4-5, 28.02.2010, p. 231-236.

Research output: Contribution to journalArticle

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