Abstract
Obesity is associated with an increased risk of breast cancer among postmenopausal women. This is at least partly due to excessive estrogen production in adipose tissue of obese women. Aromatase, the key enzyme in estrogen biosynthesis, is an important target in endocrine therapy for estrogen receptor (ER)-positive postmenopausal breast cancer. In this study we show that high confluency of human adipose stromal cells (ASCs) cultured in vitro can significantly stimulate aromatase gene expression and reduce the expression of breast tumor suppressor BRCA1 and members of the NR4A orphan nuclear family. Furthermore, small interfering RNA (siRNA)-mediated knockdown of Nurr1, a member of the NR4A family, substantially increased aromatase expression. Lastly,wefound that the cell density-triggered inducibility of aromatase expression varies in ASCs isolated from different disease-free individuals. Our finding highlights the impact of increased cell number on estrogen biosynthesis as in the case of excessive adiposity.
| Original language | English (US) |
|---|---|
| Pages (from-to) | 231-236 |
| Number of pages | 6 |
| Journal | Journal of Steroid Biochemistry and Molecular Biology |
| Volume | 118 |
| Issue number | 4-5 |
| DOIs | |
| State | Published - Feb 28 2010 |
Fingerprint
Keywords
- Adipose stromal cells
- Aromatase
- Cell density
- NR4A
ASJC Scopus subject areas
- Molecular Medicine
- Endocrinology, Diabetes and Metabolism
- Molecular Biology
- Cell Biology
- Endocrinology
- Clinical Biochemistry
- Biochemistry
- Medicine(all)
Cite this
Cell density is a critical determinant of aromatase expression in adipose stromal cells. / Ghosh, Sagar; Hu, Yanfen; Li, Rong.
In: Journal of Steroid Biochemistry and Molecular Biology, Vol. 118, No. 4-5, 28.02.2010, p. 231-236.Research output: Contribution to journal › Article
}
TY - JOUR
T1 - Cell density is a critical determinant of aromatase expression in adipose stromal cells
AU - Ghosh, Sagar
AU - Hu, Yanfen
AU - Li, Rong
PY - 2010/2/28
Y1 - 2010/2/28
N2 - Obesity is associated with an increased risk of breast cancer among postmenopausal women. This is at least partly due to excessive estrogen production in adipose tissue of obese women. Aromatase, the key enzyme in estrogen biosynthesis, is an important target in endocrine therapy for estrogen receptor (ER)-positive postmenopausal breast cancer. In this study we show that high confluency of human adipose stromal cells (ASCs) cultured in vitro can significantly stimulate aromatase gene expression and reduce the expression of breast tumor suppressor BRCA1 and members of the NR4A orphan nuclear family. Furthermore, small interfering RNA (siRNA)-mediated knockdown of Nurr1, a member of the NR4A family, substantially increased aromatase expression. Lastly,wefound that the cell density-triggered inducibility of aromatase expression varies in ASCs isolated from different disease-free individuals. Our finding highlights the impact of increased cell number on estrogen biosynthesis as in the case of excessive adiposity.
AB - Obesity is associated with an increased risk of breast cancer among postmenopausal women. This is at least partly due to excessive estrogen production in adipose tissue of obese women. Aromatase, the key enzyme in estrogen biosynthesis, is an important target in endocrine therapy for estrogen receptor (ER)-positive postmenopausal breast cancer. In this study we show that high confluency of human adipose stromal cells (ASCs) cultured in vitro can significantly stimulate aromatase gene expression and reduce the expression of breast tumor suppressor BRCA1 and members of the NR4A orphan nuclear family. Furthermore, small interfering RNA (siRNA)-mediated knockdown of Nurr1, a member of the NR4A family, substantially increased aromatase expression. Lastly,wefound that the cell density-triggered inducibility of aromatase expression varies in ASCs isolated from different disease-free individuals. Our finding highlights the impact of increased cell number on estrogen biosynthesis as in the case of excessive adiposity.
KW - Adipose stromal cells
KW - Aromatase
KW - Cell density
KW - NR4A
UR - http://www.scopus.com/inward/record.url?scp=77950347593&partnerID=8YFLogxK
UR - http://www.scopus.com/inward/citedby.url?scp=77950347593&partnerID=8YFLogxK
U2 - 10.1016/j.jsbmb.2009.12.001
DO - 10.1016/j.jsbmb.2009.12.001
M3 - Article
C2 - 19969081
AN - SCOPUS:77950347593
VL - 118
SP - 231
EP - 236
JO - Journal of Steroid Biochemistry and Molecular Biology
JF - Journal of Steroid Biochemistry and Molecular Biology
SN - 0960-0760
IS - 4-5
ER -