Cell cycle-related transfer of proteins between the nucleus and cytoplasm of Physarum polycephalum

J. R. Jeter, I. L. Cameron, N. E. Hart, H. P. Rusch

Research output: Contribution to journalArticlepeer-review

1 Scopus citations

Abstract

The proteins of wild-type and polyploid plasmodia of P. polycephalum were prelabelled with [3H]leucine and [14C]leucine. The two types of plasmodia were then fused for 2 h. Following fusion the nuclei were isolated and the smaller wild-type cell nuclei separated from the larger polyploid cell nuclei. The proteins were isolated from the recipient cell nuclei and the recipient nuclear proteins extracted. Ratios of 3H/14C in the various nuclear protein fractions show that during fusion differential transfer of labelled preformed proteins from the donor cell into the recipient cell nucleus occurs. The quantity of proteins transferred varies among the different fractions and with the phase of the cell cycle. Isotopic dilution experiments indicate that these differences in protein transfer are, in part, due to a high rate of synthesis and turnover of the nuclear proteins.

Original languageEnglish (US)
Pages (from-to)474-480
Number of pages7
JournalExperimental Cell Research
Volume138
Issue number2
DOIs
StatePublished - Apr 1982
Externally publishedYes

ASJC Scopus subject areas

  • Cell Biology

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