Cell attachment to collagen: The ionic requirements

Robert J. Klebe, James R. Hall, Pamella Rosenberger, W. Daryl Dickey

Research output: Contribution to journalArticlepeer-review

33 Scopus citations


This study is concerned with three aspects of the ionic requirements for cell attachment to collagen; namely (a) the divalent, (b) monovalent cation specificities and (c) pH optima for cell interaction with collagen. The divalent cation requirement for cell attachment to collagen can be sufficed by Ca2+, Mg2+ and certain transition group metals; whereas Ba2+, Sr2+, and polyamines are inactive. The pH optimum for cell attachment in this system occurs in the physiological range. The monovalent cation requirement for cell attachment to collagen is satisfied by isotonic NaCl, KCl, LiCl, NH4Cl, sucrose, and glucose. Pronounced inhibition of cell attachment occurs under both hypertonic and hypotonic conditions.

Original languageEnglish (US)
Pages (from-to)419-425
Number of pages7
JournalExperimental Cell Research
Issue number2
StatePublished - Dec 1977
Externally publishedYes

ASJC Scopus subject areas

  • Cell Biology


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