Cell attachment to collagen. Isolation of a cell attachment mutant

R. J. Klebe, P. G. Rosenberger, S. L. Naylor, R. L. Burns, R. Novak, H. Kleinman

Research output: Contribution to journalArticlepeer-review

24 Scopus citations

Abstract

A cell attachment mutant of the Chinese hamster ovary cell line (CHO) was isolated with the aid of an enrichment procedure. The mutant isolated, CHOatt-, was found to be defective in its attachment to collagen in two respects. (1) The mutant requires 100- and 1 000-fold more Mg2+ and Ca2+, respectively, than wild-type cells for 50% cell attachment to collagen; (2) the mutant displays only 5% of the affinity of wild-type cells for a serum cell attachment protein. CHOatt-, which was selected for non-attachment to collagen, is also altered in its ability to attach to synthetic substrata, such as glass and plastic. Collagen adhesive revertants of CHOatt- were isolated. The revertants differed morphologically from wild type CHO cells. The adhesive response to Ca2+, Mg2+, and cell attachment protein in each revertant was either partially or fully restored.

Original languageEnglish (US)
Pages (from-to)119-125
Number of pages7
JournalExperimental Cell Research
Volume104
Issue number1
DOIs
StatePublished - Jan 1977
Externally publishedYes

ASJC Scopus subject areas

  • Cell Biology

Fingerprint

Dive into the research topics of 'Cell attachment to collagen. Isolation of a cell attachment mutant'. Together they form a unique fingerprint.

Cite this