C/EBP and C-Myb sites are important for the functional activity of the human myeloperoxidase upstream enhancer

Congjun Yao, Zhenyu Qin, Kimberly N. Works, Garth E. Austin, Andrew N. Young

Research output: Contribution to journalArticle

9 Scopus citations

Abstract

Myeloperoxidase (MPO), an enzyme active against bacterial and fungal infections, is expressed specifically in myeloblasts and promyelocytes and minimal in other cell types. We recently identified and partially characterized an upstream enhancer located between -4100 and -3844 bp of the MPO gene. We showed that an AML1 site contributes to enhancer activity and specificity. We now demonstrate three additional footprints within the MPO enhancer and provide evidence that C/EBP and c-Myb sites contribute to its functional, tissue-specific activity. This distal enhancer appears to play an important role in the control of MPO transcription during differentiation of myeloid cells.

Original languageEnglish (US)
Pages (from-to)309-314
Number of pages6
JournalBiochemical and Biophysical Research Communications
Volume371
Issue number2
DOIs
StatePublished - Jun 27 2008

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Keywords

  • C/EBP
  • Enhancer
  • Myeloperoxidase
  • Promoter
  • c-Myb

ASJC Scopus subject areas

  • Biophysics
  • Biochemistry
  • Molecular Biology
  • Cell Biology

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