cDNA microarray technology and its applications

Charlie C. Xiang, Yidong Chen

Research output: Contribution to journalReview article

61 Scopus citations

Abstract

The cDNA microarray is the most powerful tool for studying gene expression in many different organisms. It has been successfully applied to the simultaneous expression of many thousands of genes and to large-scale gene discovery, as well as polymorphism screening and mapping of genomic DNA clones. It is a high throughput, highly parallel RNA expression assay technique that permits quantitative analysis of RNAs transcribed from both known and unknown genes. This technique provides diagnostic fingerprints by comparing gene expression patterns in normal and pathological cells, and because it can simultaneously track expression levels of many genes, it provides a source of operational context for inference and predication about complex cell control systems. This review describes this recently developed cDNA microarray technology and its application to gene discovery and expression, and to diagnostics for certain diseases. (C) 2000 Published by Elsevier Science Inc.

Original languageEnglish (US)
Pages (from-to)35-46
Number of pages12
JournalBiotechnology Advances
Volume18
Issue number1
DOIs
StatePublished - Mar 1 2000
Externally publishedYes

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Keywords

  • RNA expression assay technique
  • Technology
  • cDNA microarray

ASJC Scopus subject areas

  • Biotechnology
  • Bioengineering
  • Applied Microbiology and Biotechnology

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