CDC42HS: The human homolog of a yeast cell-division cycle protein

Matthew J Hart, David Leonard, Katsuhiro Shinjo, Tony Evans, Richard A. Cerione

Research output: Chapter in Book/Report/Conference proceedingChapter

Abstract

One of the first RAS-related proteins to be identified and purified was a 22-kDa protein, designated Gp (also later called G25K). Initial isolation was based on the ability of the protein to bind radiolabeled guanine nucleotides and utilized a detergent extract of a human placental particulate fraction as a source. 1 Subsequently, the same or very similar protein was identified and purified from detergent extracts of human platelet 2 and bovine brain membranes. 3 Primary amino acid sequence obtained from peptide fragments derived from both the human placental and platelet preparations demonstrated the existence of highly conserved regions implicated in GTP-binding/GTP hydrolysis and allowed for the generation of highly specific antipeptide sera. 4 The availability of primary sequence also allowed for the cloning of the cDNA's encoding this RAS-related protein. 5, 6 Here we will focus on recent studies suggesting a link between this protein and signaling pathways activated by receptor tyrosine kinases and also the information currently available on the protein-protein interactions that serve to regulate their GDP-binding/GTPase cycle.

Original languageEnglish (US)
Title of host publicationThe ras Superfamily of GTPases
PublisherCRC Press
Pages313-328
Number of pages16
ISBN (Electronic)9781351356855
ISBN (Print)9781138105126
DOIs
StatePublished - Jan 1 2017
Externally publishedYes

ASJC Scopus subject areas

  • Biochemistry, Genetics and Molecular Biology(all)
  • Medicine(all)

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  • Cite this

    Hart, M. J., Leonard, D., Shinjo, K., Evans, T., & Cerione, R. A. (2017). CDC42HS: The human homolog of a yeast cell-division cycle protein. In The ras Superfamily of GTPases (pp. 313-328). CRC Press. https://doi.org/10.1201/9780203709931