CD8α+ dendritic cells improve collagen-induced arthritis in CC chemokine receptor (CCR)-2 deficient mice

Jessica M. Ibarra, Marlon P. Quinones, Carlos A. Estrada, Fabio Jimenez, Hernan G. Martinez, Seema S. Ahuja

Research output: Contribution to journalArticle

4 Scopus citations

Abstract

Objective: Dendritic cells (DCs) have long been recognized as potential therapeutic targets of rheumatoid arthritis (RA). Increasing evidence has showed that DCs are capable of suppressing autoimmunity by expanding FoxP3+ regulatory T cells (Treg), which in turn exert immunosuppression by increasing TGFβ-1. In the SKG mice, activated DC prime autoreactive T cells causing autoantibody production and an inflammatory arthritic response. Recently, we reported that CC-chemokine receptor-2 deficient (Ccr2-/-) mice had impaired DCs migration and reduced CD8α+ DCs in the C57Bl/6J mice strain and that these mice were more susceptible to collagen antibody-induced arthritis (CAIA), compared to wild type mice. To examine the mechanism by which DCs contribute to the increased susceptibility of arthritis in Ccr2-/- mice, we tested the hypothesis that CD8α+ DCs are protective (tolerogenic) against autoimmune arthritis by examining the role of CD8α+ DCs in Ccr2-/- and SKG mice. Methods: To examine the mechanism by which DCs defects lead to the development of arthritis, we used two murine models of experimental arthritis: collagen-induced arthritis (CIA) in DBA1/J mice and zymosan-induced arthritis in SKG mice. DBA1/J mice received recombinant fms-like tyrosine kinase 3 ligand (Flt3L) injections to expand endogenous DCs populations or adoptive transfers of CD8α+ DCs. Results: Flt3L-mediated expansion of endogenous CD8α+ DCs resulted in heightened susceptibility of CIA. In contrast, supplementation with exogenous CD8α+ DCs ameliorated arthritis in Ccr2-/- mice and enhanced TGFβ1 production by T cells. Furthermore, SKG mice with genetic inactivation of CCR2 did not affect the numbers of DCs nor improve the arthritis phenotype. Conclusion: CD8α+ DCs were tolerogenic to the development of arthritis. CD8α+ DCs deficiency heightened the sensitivity to arthritis in Ccr2-/- mice. Ccr2 deficiency did not alter the arthritic phenotype in SKG mice suggesting the arthritis in Ccr2-/- mice was T cell-independent.

Original languageEnglish (US)
Pages (from-to)971-978
Number of pages8
JournalImmunobiology
Volume216
Issue number9
DOIs
StatePublished - Sep 1 2011

Keywords

  • Arthritis
  • CCR2
  • CD8α DC
  • Dendritic cells
  • SKG

ASJC Scopus subject areas

  • Immunology and Allergy
  • Immunology
  • Hematology

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