CD4-immunoglobulin G2 protects Hu-PBL-SCID mice against challenge by primary human immunodeficiency virus type 1 isolates

Marie Claire Gauduin, Graham P. Allaway, William C. Olson, Raymond Weir, Paul J. Maddon, Richard A. Koup

Research output: Contribution to journalArticle

39 Citations (Scopus)

Abstract

CD4-immunoglobulin G2 (lgG2) is a fusion protein comprising human IgG2 in which the Fv portions of both heavy and light chains have been replaced by the V1 and V2 domains of human CD4. Previous studies found that CD4-IgG2 potently neutralizes a broad range of primary human immunodeficiency virus type 1 (HIV- 1) isolates in vitro and ex vivo. The current report demonstrates that CD4-IgG2 protects against infection by primary isolates of HIV-1 in vivo, using the hu-PBL-SCID mouse model. Passive administration of 10 mg of CD4-IgG2 per kg of body weight protected all animals against subsequent challenge with 10 mouse infectious doses of the laboratory- adapted T-cell-tropic isolate HIV-l(LAI), while 50 mg of CD4-IgG2 per kg protected four of five mice against the primary isolates HIV-1(JR-CSF) and HIV-l(AD6). In contrast, a polyclonal HIV-1 Ig fraction exhibited partial protection against HIV-1(LAI) at 150 mg/kg but no significant protection against the primary HIV-1 isolates. The results demonstrate that CD4-1gG2 effectively neutralizes primary HIV-1 isolates in vivo and can prevent the initiation of infection by these viruses.

Original languageEnglish (US)
Pages (from-to)3475-3478
Number of pages4
JournalJournal of Virology
Volume72
Issue number4
StatePublished - Apr 1998
Externally publishedYes

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SCID Mice
Human immunodeficiency virus 1
immunoglobulins
HIV-1
Immunoglobulins
mice
HIV
Virus Diseases
infection
tropics
T-lymphocytes
Immunoglobulin G
animal models
Body Weight
T-Lymphocytes
Light
viruses
body weight
CD4-IgG(2)
dosage

ASJC Scopus subject areas

  • Immunology

Cite this

Gauduin, M. C., Allaway, G. P., Olson, W. C., Weir, R., Maddon, P. J., & Koup, R. A. (1998). CD4-immunoglobulin G2 protects Hu-PBL-SCID mice against challenge by primary human immunodeficiency virus type 1 isolates. Journal of Virology, 72(4), 3475-3478.

CD4-immunoglobulin G2 protects Hu-PBL-SCID mice against challenge by primary human immunodeficiency virus type 1 isolates. / Gauduin, Marie Claire; Allaway, Graham P.; Olson, William C.; Weir, Raymond; Maddon, Paul J.; Koup, Richard A.

In: Journal of Virology, Vol. 72, No. 4, 04.1998, p. 3475-3478.

Research output: Contribution to journalArticle

Gauduin, MC, Allaway, GP, Olson, WC, Weir, R, Maddon, PJ & Koup, RA 1998, 'CD4-immunoglobulin G2 protects Hu-PBL-SCID mice against challenge by primary human immunodeficiency virus type 1 isolates', Journal of Virology, vol. 72, no. 4, pp. 3475-3478.
Gauduin, Marie Claire ; Allaway, Graham P. ; Olson, William C. ; Weir, Raymond ; Maddon, Paul J. ; Koup, Richard A. / CD4-immunoglobulin G2 protects Hu-PBL-SCID mice against challenge by primary human immunodeficiency virus type 1 isolates. In: Journal of Virology. 1998 ; Vol. 72, No. 4. pp. 3475-3478.
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