CD30 is a CD40-inducible molecule that negatively regulates CD40- mediated immunoglobulin class switching in non-antigen-selected human B cells

Andrea Cerutti, Andrés Schaffer, Shefali Shah, Hong Zan, Hsiou Chi Liou, Raymond G. Goodwin, Paolo Casali

Research output: Contribution to journalArticle

63 Citations (Scopus)

Abstract

We used our monoclonal model of germinal center maturation, CL-01 B cells, to investigate the role of CD30 in human B cell differentiation. CL- 01 cells are IgM+IgD+CD30+ and switch to IgG, IgA, and IgE when exposed to CD40L and IL-4. Switching is hampered by CD30 coengagement, possibly through interference with the CD40-mediated NF-κB-dependent transcriptional activation of downstream C(H) genes. The physiological relevance of this phenomenon is emphasized by similar CD30-mediated effects in naive B cells. Expression of CD30 by these cells is induced by CD40L but is inhibited by B cell receptor coengagement and/or exposure to IL-6 and IL-12. Our data suggest that CD30 critically regulates the CD40-mediated differentiation of non-antigen-selected human B cells.

Original languageEnglish (US)
Pages (from-to)247-256
Number of pages10
JournalImmunity
Volume9
Issue number2
DOIs
StatePublished - Jan 1 1998
Externally publishedYes

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Immunoglobulin Class Switching
B-Lymphocytes
CD40 Ligand
Physiological Phenomena
Immunoglobulin D
Germinal Center
Differentiation Antigens
Interleukin-12
Interleukin-4
Immunoglobulin A
Immunoglobulin E
Transcriptional Activation
Immunoglobulin M
Cell Differentiation
Interleukin-6
Immunoglobulin G
Genes

ASJC Scopus subject areas

  • Immunology and Allergy
  • Immunology
  • Infectious Diseases

Cite this

CD30 is a CD40-inducible molecule that negatively regulates CD40- mediated immunoglobulin class switching in non-antigen-selected human B cells. / Cerutti, Andrea; Schaffer, Andrés; Shah, Shefali; Zan, Hong; Liou, Hsiou Chi; Goodwin, Raymond G.; Casali, Paolo.

In: Immunity, Vol. 9, No. 2, 01.01.1998, p. 247-256.

Research output: Contribution to journalArticle

Cerutti, Andrea ; Schaffer, Andrés ; Shah, Shefali ; Zan, Hong ; Liou, Hsiou Chi ; Goodwin, Raymond G. ; Casali, Paolo. / CD30 is a CD40-inducible molecule that negatively regulates CD40- mediated immunoglobulin class switching in non-antigen-selected human B cells. In: Immunity. 1998 ; Vol. 9, No. 2. pp. 247-256.
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AU - Shah, Shefali

AU - Zan, Hong

AU - Liou, Hsiou Chi

AU - Goodwin, Raymond G.

AU - Casali, Paolo

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