CD30 is a CD40-inducible molecule that negatively regulates CD40- mediated immunoglobulin class switching in non-antigen-selected human B cells

Andrea Cerutti; Andrés Schaffer; Shefali Shah; Hong Zan; Hsiou Chi Liou; Raymond G. Goodwin; Paolo Casali

doi:10.1016/S1074-7613(00)80607-X

CD30 is a CD40-inducible molecule that negatively regulates CD40- mediated immunoglobulin class switching in non-antigen-selected human B cells

Andrea Cerutti, Andrés Schaffer, Shefali Shah, Hong Zan, Hsiou Chi Liou, Raymond G. Goodwin, Paolo Casali

Research output: Contribution to journal › Article › peer-review

66 Scopus citations

Abstract

We used our monoclonal model of germinal center maturation, CL-01 B cells, to investigate the role of CD30 in human B cell differentiation. CL- 01 cells are IgM⁺IgD⁺CD30⁺ and switch to IgG, IgA, and IgE when exposed to CD40L and IL-4. Switching is hampered by CD30 coengagement, possibly through interference with the CD40-mediated NF-κB-dependent transcriptional activation of downstream C(H) genes. The physiological relevance of this phenomenon is emphasized by similar CD30-mediated effects in naive B cells. Expression of CD30 by these cells is induced by CD40L but is inhibited by B cell receptor coengagement and/or exposure to IL-6 and IL-12. Our data suggest that CD30 critically regulates the CD40-mediated differentiation of non-antigen-selected human B cells.

Original language	English (US)
Pages (from-to)	247-256
Number of pages	10
Journal	Immunity
Volume	9
Issue number	2
DOIs	https://doi.org/10.1016/S1074-7613(00)80607-X
State	Published - Aug 1998
Externally published	Yes

ASJC Scopus subject areas

Infectious Diseases
Immunology and Allergy
Immunology

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10.1016/S1074-7613(00)80607-X

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title = "CD30 is a CD40-inducible molecule that negatively regulates CD40- mediated immunoglobulin class switching in non-antigen-selected human B cells",

abstract = "We used our monoclonal model of germinal center maturation, CL-01 B cells, to investigate the role of CD30 in human B cell differentiation. CL- 01 cells are IgM+IgD+CD30+ and switch to IgG, IgA, and IgE when exposed to CD40L and IL-4. Switching is hampered by CD30 coengagement, possibly through interference with the CD40-mediated NF-κB-dependent transcriptional activation of downstream C(H) genes. The physiological relevance of this phenomenon is emphasized by similar CD30-mediated effects in naive B cells. Expression of CD30 by these cells is induced by CD40L but is inhibited by B cell receptor coengagement and/or exposure to IL-6 and IL-12. Our data suggest that CD30 critically regulates the CD40-mediated differentiation of non-antigen-selected human B cells.",

author = "Andrea Cerutti and Andr{\'e}s Schaffer and Shefali Shah and Hong Zan and Liou, {Hsiou Chi} and Goodwin, {Raymond G.} and Paolo Casali",

note = "Funding Information: Address all correspondence to P. C. We are grateful to Dr. B. Perussia (Thomas Jefferson Cancer Center, Philadelphia, PA) for human recombinant IL-12 and mouse neutralizing Abs to human IFNγ. We thank Ms. P. Dramitinos for her skillful technical assistance. This work was supported by U. S. Public Health Service grants AR 40908 and CA 68541 to P. C.",

year = "1998",

month = aug,

doi = "10.1016/S1074-7613(00)80607-X",

language = "English (US)",

volume = "9",

pages = "247--256",

journal = "Immunity",

issn = "1074-7613",

publisher = "Cell Press",

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TY - JOUR

T1 - CD30 is a CD40-inducible molecule that negatively regulates CD40- mediated immunoglobulin class switching in non-antigen-selected human B cells

AU - Cerutti, Andrea

AU - Schaffer, Andrés

AU - Shah, Shefali

AU - Zan, Hong

AU - Liou, Hsiou Chi

AU - Goodwin, Raymond G.

AU - Casali, Paolo

N1 - Funding Information: Address all correspondence to P. C. We are grateful to Dr. B. Perussia (Thomas Jefferson Cancer Center, Philadelphia, PA) for human recombinant IL-12 and mouse neutralizing Abs to human IFNγ. We thank Ms. P. Dramitinos for her skillful technical assistance. This work was supported by U. S. Public Health Service grants AR 40908 and CA 68541 to P. C.

PY - 1998/8

Y1 - 1998/8

N2 - We used our monoclonal model of germinal center maturation, CL-01 B cells, to investigate the role of CD30 in human B cell differentiation. CL- 01 cells are IgM+IgD+CD30+ and switch to IgG, IgA, and IgE when exposed to CD40L and IL-4. Switching is hampered by CD30 coengagement, possibly through interference with the CD40-mediated NF-κB-dependent transcriptional activation of downstream C(H) genes. The physiological relevance of this phenomenon is emphasized by similar CD30-mediated effects in naive B cells. Expression of CD30 by these cells is induced by CD40L but is inhibited by B cell receptor coengagement and/or exposure to IL-6 and IL-12. Our data suggest that CD30 critically regulates the CD40-mediated differentiation of non-antigen-selected human B cells.

AB - We used our monoclonal model of germinal center maturation, CL-01 B cells, to investigate the role of CD30 in human B cell differentiation. CL- 01 cells are IgM+IgD+CD30+ and switch to IgG, IgA, and IgE when exposed to CD40L and IL-4. Switching is hampered by CD30 coengagement, possibly through interference with the CD40-mediated NF-κB-dependent transcriptional activation of downstream C(H) genes. The physiological relevance of this phenomenon is emphasized by similar CD30-mediated effects in naive B cells. Expression of CD30 by these cells is induced by CD40L but is inhibited by B cell receptor coengagement and/or exposure to IL-6 and IL-12. Our data suggest that CD30 critically regulates the CD40-mediated differentiation of non-antigen-selected human B cells.

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CD30 is a CD40-inducible molecule that negatively regulates CD40- mediated immunoglobulin class switching in non-antigen-selected human B cells

Abstract

ASJC Scopus subject areas

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