CD20 (pan-B cell antigen) expression on bone marrow-derived T cells

Kenneth M. Algino, Ronald W. Thomason, David E. King, Milka M. Montiel, Fiona E. Craig

Research output: Contribution to journalArticlepeer-review

52 Scopus citations

Abstract

Antibodies directed against CD20 (L26, Leu 16, and B1) are frequently used to determine the presence of B lymphocytes. However, recent publications describe the unexpected presence of CD20-positive T cells in the peripheral blood of normal subjects and occasional T-cell neoplasms that express CD20. To determine the presence of CD20-positive T cells in bone marrow, flow cytometric analysis was performed on 34 aspirate specimens (14 normal, 5 acute lymphoblastic lymphoma [ALL], 5 acute myelogenous leukemia [AML], 4 HIV positive, 2 myelodysplastic/myeloproliferative, 2 chronic myelogenous leukemia [CML], 1 chronic lymphocytic lymphoma [CLL], 1 multiple myeloma). A small population of cells coexpressing CD3 (Leu 4) and CD20(dim) (Leu 16) was identified in 94% of the specimens, representing 0% to 11% (mean 1.77%) of marrow mononuclear cells and 0% to 22.2% (mean 6.54%) of marrow lymphoid cells. There was no correlation between the percentage of CD20-positive T cells and the CD4:CD8 ratio, patient age, gender, or diagnosis. CD20(dim), positive cells included immature B cells and CD20-positive T cells. Although evaluation of CD20 expression is useful in delineating B-cell processes, caution should be exercised in interpreting its expression on bone marrow T- lymphoid cells. CD20 expression on T cells may be seen in either normal, reactive, or neoplastic processes.

Original languageEnglish (US)
Pages (from-to)78-81
Number of pages4
JournalAmerican journal of clinical pathology
Volume106
Issue number1
DOIs
StatePublished - Jul 1996
Externally publishedYes

Keywords

  • Hematologic diseases
  • Hematopoietic differentiation antigens
  • Immunophenotyping
  • Lymphocytes

ASJC Scopus subject areas

  • Pathology and Forensic Medicine

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