Catalysis of guanine nucleotide exchange on the CDC42Hs protein by the dbl oncogene product

Matthew J Hart, Alessandra Eva, Tony Evans, Stuart A. Aaronson, Richard A. Cerione

Research output: Contribution to journalArticle

320 Citations (Scopus)

Abstract

THE superfamily of low molecular mass GTP-binding proteins, for which the ras proteins are prototypes, has been implicated in the regulation of diverse biological activities including protein trafficking, secretion, and cell growth and differentiation1-3. One member of this family, CDC42Hs (originally referred to as Gp or G25K)4,5, seems to be the human homologue of the Saccharomyces cerevisiae cell-division-cycle protein, CDC42Sc (refs 6-9). A second S. cerevisiae protein, CDC24 (ref. 10), which is known from complementation studies to act with CDC42Sc to regulate the development of normal cell shape and the selection of nonrandom budding sites in yeast, contains a region with sequence similarity to the dbl oncogene product11-14. Here we show that dbl specifically catalyses the dissociation of GDP from CDC42Hs and thereby qualifies as a highly selective guanine nucleotide exchange factor for the GTP-binding protein. Although guanine nucleotide exchange activities have been previously described for other members of the Ras-related GTP-binding protein family15-17, this is the first demonstration, to our knowledge, of the involvement of a human oncogenic protein in catalysing exchange activity.

Original languageEnglish (US)
Pages (from-to)311-314
Number of pages4
JournalNature
Volume354
Issue number6351
StatePublished - Nov 28 1991
Externally publishedYes

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Guanine Nucleotides
Oncogene Proteins
Catalysis
GTP-Binding Proteins
Saccharomyces cerevisiae Proteins
Guanine Nucleotide Exchange Factors
ras Proteins
Cell Cycle Proteins
Monomeric GTP-Binding Proteins
Cell Shape
Protein Transport
Oncogenes
Proteins
Yeasts
Growth
S cerevisiae CDC24 protein

ASJC Scopus subject areas

  • General

Cite this

Hart, M. J., Eva, A., Evans, T., Aaronson, S. A., & Cerione, R. A. (1991). Catalysis of guanine nucleotide exchange on the CDC42Hs protein by the dbl oncogene product. Nature, 354(6351), 311-314.

Catalysis of guanine nucleotide exchange on the CDC42Hs protein by the dbl oncogene product. / Hart, Matthew J; Eva, Alessandra; Evans, Tony; Aaronson, Stuart A.; Cerione, Richard A.

In: Nature, Vol. 354, No. 6351, 28.11.1991, p. 311-314.

Research output: Contribution to journalArticle

Hart, MJ, Eva, A, Evans, T, Aaronson, SA & Cerione, RA 1991, 'Catalysis of guanine nucleotide exchange on the CDC42Hs protein by the dbl oncogene product', Nature, vol. 354, no. 6351, pp. 311-314.
Hart MJ, Eva A, Evans T, Aaronson SA, Cerione RA. Catalysis of guanine nucleotide exchange on the CDC42Hs protein by the dbl oncogene product. Nature. 1991 Nov 28;354(6351):311-314.
Hart, Matthew J ; Eva, Alessandra ; Evans, Tony ; Aaronson, Stuart A. ; Cerione, Richard A. / Catalysis of guanine nucleotide exchange on the CDC42Hs protein by the dbl oncogene product. In: Nature. 1991 ; Vol. 354, No. 6351. pp. 311-314.
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