Catalase transgenic mice: Characterization and sensitivity to oxidative stress

Xinlian Chen, Hanyu Liang, Holly Van Remmen, Jan Vijg, Arlan Richardson

Research output: Contribution to journalArticle

60 Scopus citations

Abstract

The role of catalase in the antioxidant defense system was studied using transgenic mice [Tg(CAT)] harboring a human genomic clone containing the entire human CAT gene. Catalase activity was 2-fold higher in the tissues of hemizygous [Tg(CAT)+/o] mice and 3- to 4-fold higher in the tissues of homozygous [Tg(CAT)+/+] mice compared to wild type mice. The human CAT transgene was expressed in a tissue-specific pattern that was similar to the endogenous catalase gene. The levels of other major antioxidant enzymes were not altered in the tissues of the transgenic mice. Hepatocytes and fibroblasts from the Tg(CAT)+/+ mice were more resistant to hydrogen peroxide-induced cell death but were more sensitive to paraquat and TNFα toxicity. Fibroblasts from the Tg(CAT)+/+ mice showed reduced growth rate in culture without treatment and reduced colony-forming capability after γ-irradiation compared to fibroblasts from wild type mice. In addition, the Tg(CAT)+/+ animals were more sensitive to γ-irradiation.

Original languageEnglish (US)
Pages (from-to)197-210
Number of pages14
JournalArchives of Biochemistry and Biophysics
Volume422
Issue number2
DOIs
StatePublished - Feb 15 2004

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Keywords

  • Antioxidant enzymes
  • Catalase
  • Oxidative stress
  • Transgenic mice

ASJC Scopus subject areas

  • Biophysics
  • Biochemistry
  • Molecular Biology

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