[Ca2++Mg2+]-dependent ATPase activity in rat pineal gland

Li Dun Chen, Lucien C. Manchester, Russel J Reiter, Dun Xian Tan, Burkhard Poeggeler

Research output: Contribution to journalArticle

4 Citations (Scopus)

Abstract

[Ca2++Mg2+]-dependent ATPase activity in the pineal gland of the rat was examined. The enzyme possesses an apparent Km (Ca2+) of 0.23 μM, and moderately high affinity for Mg2+ and ATP (Km = 53.2 μM and Km = 86.4 μM, respectively). The ATPase activity is sensitive to low concentrations (I50 approximately 1 μM) of vanadate, which specifically inhibits Ca2+-ATPase in the plasma membranes of the erythrocyte, cardiomyocytes and synapses. The calmodulin antagonist trifluoperazine reduced significantly Ca2+-stimulated, Mg2+-dependent ATP hydrolysis. The [Ca2++Mg2+]-dependent ATPase in rat pineal gland exhibits very high affinity for Ca2+, is highly vanadate sensitive and appears to require calmodulin. The enzyme is similar to the Ca2+-ATPase of the erythrocyte, cardiomyocytes and synaptic plasma membranes. These new findings may help to elucidate the mechanisms of intracellular calcium homeostasis and the effect of the enzyme on the synthesis of melatonin in the pineal gland.

Original languageEnglish (US)
Pages (from-to)131-134
Number of pages4
JournalNeuroscience Letters
Volume157
Issue number2
DOIs
StatePublished - Jul 23 1993

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Ca(2+) Mg(2+)-ATPase
Pineal Gland
Vanadates
Calcium-Transporting ATPases
Calmodulin
Cardiac Myocytes
Enzymes
Adenosine Triphosphate
Erythrocytes
Cell Membrane
Trifluoperazine
Synaptic Membranes
Melatonin
Synapses
Adenosine Triphosphatases
Hydrolysis
Homeostasis
Calcium

Keywords

  • Ca-ATPase
  • Calcium
  • Pineal gland
  • Rat

ASJC Scopus subject areas

  • Neuroscience(all)

Cite this

[Ca2++Mg2+]-dependent ATPase activity in rat pineal gland. / Chen, Li Dun; Manchester, Lucien C.; Reiter, Russel J; Tan, Dun Xian; Poeggeler, Burkhard.

In: Neuroscience Letters, Vol. 157, No. 2, 23.07.1993, p. 131-134.

Research output: Contribution to journalArticle

Chen, LD, Manchester, LC, Reiter, RJ, Tan, DX & Poeggeler, B 1993, '[Ca2++Mg2+]-dependent ATPase activity in rat pineal gland', Neuroscience Letters, vol. 157, no. 2, pp. 131-134. https://doi.org/10.1016/0304-3940(93)90720-6
Chen, Li Dun ; Manchester, Lucien C. ; Reiter, Russel J ; Tan, Dun Xian ; Poeggeler, Burkhard. / [Ca2++Mg2+]-dependent ATPase activity in rat pineal gland. In: Neuroscience Letters. 1993 ; Vol. 157, No. 2. pp. 131-134.
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