Ca2+ currents of fast blue-labeled superior cervical ganglion neurons

Chu Chen, Geoffrey G. Schofield

Research output: Contribution to journalArticlepeer-review

8 Scopus citations


To investigated if Ca2+ currents of acutely isolated superior cervical ganglion (SCG) neurons are altered after dye labeling or ultraviolet (UV) exposure, SCG neurons were labeled by multiple injections of 2% fast blue (FB) (S-μl total) into the submaxillary gland. Ca2+ currents of both labeled and unlabeled neurons were significantly depressed by 2-min exposure to UV, compared to labeled and unlabeled neurons not exposed to UV. The I-V curve of labeled neurons was shifted hyperpolarized after UV exposure. Tail-current activation curves of both labeled and unlabeled neurons were fitted to a double Boltzmann equation. In labeled neurons, 2-min exposure to UV produced a significant reduction of the amplitude of the more depolarized component as well as a hyperpolarizing shift of the half activation potential of both components of the activation curve. However, the short time (< 10 s) required to identify a target-specific neuron had no significant effect on the biophysical properties of Ca2+ currents of FB-labeled SCG neurons. Thus, use of this label in combination with the patch-clamp technique comprises a powerful approach to study membrane currents of target-specific neurons.

Original languageEnglish (US)
Pages (from-to)63-69
Number of pages7
JournalJournal of Neuroscience Methods
Issue number1-2
StatePublished - 1992
Externally publishedYes


  • Calcium current
  • Fluorescent dye
  • Ion channel
  • Superior cervical ganglion
  • Sympathetic neuron
  • Ultraviolet
  • Voltage-clamp

ASJC Scopus subject areas

  • General Neuroscience


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