Ca2+ and pH modulate alternative splicing of exon 5 in NMDA receptor subunit 1

Mary Lou Vallano, Carol M. Beaman-Hall, Saloua Benmansour

Research output: Contribution to journalArticlepeer-review

20 Scopus citations

Abstract

RT-PCR and intracellular Ca2+ measurements were used to identify factors that modulate alternative splicing of exon 5 in the NMDA receptor transcript encoding NR1, in cultured cerebellar granule neurons. Although cells grown in media containing 5 mM KCl demonstrate compromised survival they show the predicted developmental transition from NR1a (-exon 5) to NR1b (+exon 5) mRNA expression. This transition was blocked under culture conditions that promote survival; inclusion or exclusion of exon 5 is a reversible process that is sensitive to alterations in Ca2+ and pH. We conclude that alternative splicing of NR1 pre-mRNA transcripts may be regulated by developmental cues that modulate the degree of glutamate receptor activation.

Original languageEnglish (US)
Pages (from-to)3659-3664
Number of pages6
JournalNeuroReport
Volume10
Issue number17
DOIs
StatePublished - Nov 26 1999

Keywords

  • Alternative splicing
  • Calcium
  • Cerebellum
  • Granule neurons
  • NMDA receptor

ASJC Scopus subject areas

  • Neuroscience(all)

Fingerprint Dive into the research topics of 'Ca<sup>2+</sup> and pH modulate alternative splicing of exon 5 in NMDA receptor subunit 1'. Together they form a unique fingerprint.

Cite this