Ca2+-activated Cl- channel currents in rat ventral prostate epithelial cells

Sung Joon Kim, Sun Young Shin, Ji Eun Lee, Jun Hee Kim, Dae Yong Uhm

Research output: Contribution to journalArticlepeer-review

24 Scopus citations

Abstract

BACKGROUND. In many epithelial tissues, the Cl- efflux via Ca2+-activated Cl- channels (ClCa) play a key role for the fluid secretion. To elucidate the mechanism of prostatic fluid secretion, the properties of whole-cell chloride conductance were investigated. MATERIALS AND METHODS. Rat prostate secretory epithelial cells (RPSECs) were isolated by collagenase treatment, and were used for the whole-cell voltage clamp. Both extra- and intracellular monovalent cations were replaced by N-methyl-D-glucamate to record the Cl- current selectively. RESULTS. A bath application of Ca2+-ionophore, ionomycin (0.2 μM), increased the membrane conductance with outwardly rectifying voltage-dependence. On step-like depolarization from -60 to +80 mV (500 msec), the ionomycin-induced current showed slowly activating kinetics, a known property of ClCa current (ICl(Ca)) of other tissues. The relative permeability of ClCa to various anions was calculated from the reversal potentials measured under a total replacement of extracellular Cl- with various anions, and the relative order of permeability was SCN- > I- > Br- > Cl- ≫ gluconate. The amplitude of ICl(Ca) was decreased by various anion channel blockers: niflumic acid (100 μM), DPC (100 μM), DIDS (1 mM), and NPPB (200 μM). CONCLUSIONS. RPSECs have ClCa that may provide Cl- efflux pathways for the exocrine secretions of the prostate.

Original languageEnglish (US)
Pages (from-to)118-127
Number of pages10
JournalProstate
Volume55
Issue number2
DOIs
StatePublished - May 1 2003
Externally publishedYes

Keywords

  • Ca-activated Cl channel
  • Prostate epithelium
  • Voltage clamp

ASJC Scopus subject areas

  • Oncology
  • Urology

Fingerprint Dive into the research topics of 'Ca<sup>2+</sup>-activated Cl<sup>-</sup> channel currents in rat ventral prostate epithelial cells'. Together they form a unique fingerprint.

Cite this