The effect of castration on pituitary common α and LH-β subunit mRNA levels was examined in adult male rats in a dot-blot hybridization assay using cytosolic mRNA and 32P labelled plasmids containing cDNA encoding sequences of the subunits. Orchidectomy increased α subunit mRNA levels threefold by day 4 with no further rise at 2 months. The LH-β subunit m RNA levels increased by threefold at 2 months. Serum LH levels increased by up to fortyfold (2 months). Pituitary LH content increased by fourfold at 2 months, though was reduced at 4 days after castration. These results suggest that transcription of the α and LH-β genes are similarly regulated after castration. However the greater magnitude of serum LH increase after castration than that of both LH subunit mRNA level and the bidirectional changes in pituitary LH content implies additional translational/post-translational control of gonadotrophin biosynthesis by gonadal hormones.
|Original language||English (US)|
|Journal||Journal of Endocrinology|
|State||Published - 1985|
ASJC Scopus subject areas
- Endocrinology, Diabetes and Metabolism