Candidal antigen detection in pulmonary candidiasis

David M. Humphrey, Marc H Weiner

Research output: Contribution to journalArticle

11 Citations (Scopus)

Abstract

To better characterize pulmonary candidlasis, lung tissue samples from 58 hospital subjects were examined by immunohistocnemical analysis with antiserums to candidal mannan and cytoplasmic antigens. In nine cases of invasive pulmonary candidiasis, fungal antigens were abundant in lung specimens and were distributed in two immunohistologic patterns. Antigen dissemination beyond the lung was confirmed by detection of antigenemia by mannan radioimmunoassay in serum samples in cases of pulmonary candidiasis of each immunohistologic pattern. In contrast, minimal, focal quantities of fungal antigens were detected in lung tissue specimens from nine subjects with thrush or aspiration but without pulmonary candidiasis; no antigen was detected in 40 additional cases of noncandldal mycotlc, bacterial, viral, and parasitic pneumonias. Immunohistochemical criteria that denote invasive pulmonary candidiasis include the abundance of diffuse candidal antigens in bronchiolar and alveolar fluid and cytoplasmic staining of phagocytes. When compared with routine histologic stains, the diagnostic yield in cases of invasive pulmonary candidiasis was significantly increased by detection of candidal antigenic material. This study of 58 well-documented cases shows that immunohistochemical detection of candidal antigens facilitates the diagnosis of candidal pneumonia and distinguishes clinically significant pulmonary candidiasis from noninvasive aspiration, opportunistic colonization, or specimen contamination.

Original languageEnglish (US)
Pages (from-to)630-640
Number of pages11
JournalThe American journal of medicine
Volume74
Issue number4
DOIs
StatePublished - 1983
Externally publishedYes

Fingerprint

Candidiasis
Antigens
Lung
Invasive Candidiasis
Fungal Antigens
Mannans
Viral Pneumonia
Oral Candidiasis
Phagocytes
Radioimmunoassay
Immune Sera
Pneumonia
Coloring Agents
Staining and Labeling

ASJC Scopus subject areas

  • Nursing(all)

Cite this

Candidal antigen detection in pulmonary candidiasis. / Humphrey, David M.; Weiner, Marc H.

In: The American journal of medicine, Vol. 74, No. 4, 1983, p. 630-640.

Research output: Contribution to journalArticle

@article{cfaaf4beac9543fda3f5495042db3702,
title = "Candidal antigen detection in pulmonary candidiasis",
abstract = "To better characterize pulmonary candidlasis, lung tissue samples from 58 hospital subjects were examined by immunohistocnemical analysis with antiserums to candidal mannan and cytoplasmic antigens. In nine cases of invasive pulmonary candidiasis, fungal antigens were abundant in lung specimens and were distributed in two immunohistologic patterns. Antigen dissemination beyond the lung was confirmed by detection of antigenemia by mannan radioimmunoassay in serum samples in cases of pulmonary candidiasis of each immunohistologic pattern. In contrast, minimal, focal quantities of fungal antigens were detected in lung tissue specimens from nine subjects with thrush or aspiration but without pulmonary candidiasis; no antigen was detected in 40 additional cases of noncandldal mycotlc, bacterial, viral, and parasitic pneumonias. Immunohistochemical criteria that denote invasive pulmonary candidiasis include the abundance of diffuse candidal antigens in bronchiolar and alveolar fluid and cytoplasmic staining of phagocytes. When compared with routine histologic stains, the diagnostic yield in cases of invasive pulmonary candidiasis was significantly increased by detection of candidal antigenic material. This study of 58 well-documented cases shows that immunohistochemical detection of candidal antigens facilitates the diagnosis of candidal pneumonia and distinguishes clinically significant pulmonary candidiasis from noninvasive aspiration, opportunistic colonization, or specimen contamination.",
author = "Humphrey, {David M.} and Weiner, {Marc H}",
year = "1983",
doi = "10.1016/0002-9343(83)91021-5",
language = "English (US)",
volume = "74",
pages = "630--640",
journal = "American Journal of Medicine",
issn = "0002-9343",
publisher = "Elsevier Inc.",
number = "4",

}

TY - JOUR

T1 - Candidal antigen detection in pulmonary candidiasis

AU - Humphrey, David M.

AU - Weiner, Marc H

PY - 1983

Y1 - 1983

N2 - To better characterize pulmonary candidlasis, lung tissue samples from 58 hospital subjects were examined by immunohistocnemical analysis with antiserums to candidal mannan and cytoplasmic antigens. In nine cases of invasive pulmonary candidiasis, fungal antigens were abundant in lung specimens and were distributed in two immunohistologic patterns. Antigen dissemination beyond the lung was confirmed by detection of antigenemia by mannan radioimmunoassay in serum samples in cases of pulmonary candidiasis of each immunohistologic pattern. In contrast, minimal, focal quantities of fungal antigens were detected in lung tissue specimens from nine subjects with thrush or aspiration but without pulmonary candidiasis; no antigen was detected in 40 additional cases of noncandldal mycotlc, bacterial, viral, and parasitic pneumonias. Immunohistochemical criteria that denote invasive pulmonary candidiasis include the abundance of diffuse candidal antigens in bronchiolar and alveolar fluid and cytoplasmic staining of phagocytes. When compared with routine histologic stains, the diagnostic yield in cases of invasive pulmonary candidiasis was significantly increased by detection of candidal antigenic material. This study of 58 well-documented cases shows that immunohistochemical detection of candidal antigens facilitates the diagnosis of candidal pneumonia and distinguishes clinically significant pulmonary candidiasis from noninvasive aspiration, opportunistic colonization, or specimen contamination.

AB - To better characterize pulmonary candidlasis, lung tissue samples from 58 hospital subjects were examined by immunohistocnemical analysis with antiserums to candidal mannan and cytoplasmic antigens. In nine cases of invasive pulmonary candidiasis, fungal antigens were abundant in lung specimens and were distributed in two immunohistologic patterns. Antigen dissemination beyond the lung was confirmed by detection of antigenemia by mannan radioimmunoassay in serum samples in cases of pulmonary candidiasis of each immunohistologic pattern. In contrast, minimal, focal quantities of fungal antigens were detected in lung tissue specimens from nine subjects with thrush or aspiration but without pulmonary candidiasis; no antigen was detected in 40 additional cases of noncandldal mycotlc, bacterial, viral, and parasitic pneumonias. Immunohistochemical criteria that denote invasive pulmonary candidiasis include the abundance of diffuse candidal antigens in bronchiolar and alveolar fluid and cytoplasmic staining of phagocytes. When compared with routine histologic stains, the diagnostic yield in cases of invasive pulmonary candidiasis was significantly increased by detection of candidal antigenic material. This study of 58 well-documented cases shows that immunohistochemical detection of candidal antigens facilitates the diagnosis of candidal pneumonia and distinguishes clinically significant pulmonary candidiasis from noninvasive aspiration, opportunistic colonization, or specimen contamination.

UR - http://www.scopus.com/inward/record.url?scp=0020536287&partnerID=8YFLogxK

UR - http://www.scopus.com/inward/citedby.url?scp=0020536287&partnerID=8YFLogxK

U2 - 10.1016/0002-9343(83)91021-5

DO - 10.1016/0002-9343(83)91021-5

M3 - Article

VL - 74

SP - 630

EP - 640

JO - American Journal of Medicine

JF - American Journal of Medicine

SN - 0002-9343

IS - 4

ER -