Calorie restriction attenuates inflammatory responses to myocardial ischemia-reperfusion injury

B. Chandrasekar, James F Nelson, J. T. Colston, G. L. Freeman

Research output: Contribution to journalArticle

74 Citations (Scopus)

Abstract

The life-prolonging effects of calorie restriction (CR) may be due to reduced damage from cumulative oxidative stress. Our goal was to determine the long-term effects of moderate dietary CR on the myocardial response to reperfusion after a single episode of sublethal ischemia. Male Fisher 344 rats were fed either an ad libitum (AL) or CR (40% less calories) diet. At age 12 mo the animals were anaesthetized and subjected to thoracotomy and a 15-min left-anterior descending coronary artery occlusion. The hearts were reperfused for various periods. GSH and GSSG levels, nuclear factor-κB (NF-κB) DNA binding activity, cytokine, and antioxidant enzyme expression were assessed in the ischemic zones. Sham-operated animals served as controls. Compared with the AL diet, chronic CR limited oxidative stress as seen by rapid recovery in GSH levels in previously ischemic myocardium. CR reduced DNA binding activity of NF-κB. The κB-responsive cytokines interleukin-1β and tumor necrosis factor-α were transiently expressed in the CR group but persisted longer in the AL group. Furthermore, expression of manganese superoxide dismutase, a key antioxidant enzyme, was significantly delayed in the AL group. Collectively these data indicate that CR significantly attenuates myocardial oxidative stress and the postischemic inflammatory response.

Original languageEnglish (US)
JournalAmerican Journal of Physiology - Heart and Circulatory Physiology
Volume280
Issue number5 49-5
StatePublished - May 2001

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Myocardial Reperfusion Injury
Reperfusion Injury
Myocardial Ischemia
Oxidative Stress
Antioxidants
Cytokines
Diet
Glutathione Disulfide
Coronary Occlusion
DNA
Enzymes
Thoracotomy
Interleukin-1
Superoxide Dismutase
Reperfusion
Coronary Vessels
Myocardium
Ischemia
Tumor Necrosis Factor-alpha

Keywords

  • Cytokines
  • Energy intake
  • Free radicals
  • Glutathione

ASJC Scopus subject areas

  • Physiology
  • Physiology (medical)

Cite this

Calorie restriction attenuates inflammatory responses to myocardial ischemia-reperfusion injury. / Chandrasekar, B.; Nelson, James F; Colston, J. T.; Freeman, G. L.

In: American Journal of Physiology - Heart and Circulatory Physiology, Vol. 280, No. 5 49-5, 05.2001.

Research output: Contribution to journalArticle

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