Calorie restriction attenuates inflammatory responses to myocardial ischemia-reperfusion injury

B. Chandrasekar, J. F. Nelson, J. T. Colston, G. L. Freeman

Research output: Contribution to journalArticlepeer-review

76 Scopus citations


The life-prolonging effects of calorie restriction (CR) may be due to reduced damage from cumulative oxidative stress. Our goal was to determine the long-term effects of moderate dietary CR on the myocardial response to reperfusion after a single episode of sublethal ischemia. Male Fisher 344 rats were fed either an ad libitum (AL) or CR (40% less calories) diet. At age 12 mo the animals were anaesthetized and subjected to thoracotomy and a 15-min left-anterior descending coronary artery occlusion. The hearts were reperfused for various periods. GSH and GSSG levels, nuclear factor-κB (NF-κB) DNA binding activity, cytokine, and antioxidant enzyme expression were assessed in the ischemic zones. Sham-operated animals served as controls. Compared with the AL diet, chronic CR limited oxidative stress as seen by rapid recovery in GSH levels in previously ischemic myocardium. CR reduced DNA binding activity of NF-κB. The κB-responsive cytokines interleukin-1β and tumor necrosis factor-α were transiently expressed in the CR group but persisted longer in the AL group. Furthermore, expression of manganese superoxide dismutase, a key antioxidant enzyme, was significantly delayed in the AL group. Collectively these data indicate that CR significantly attenuates myocardial oxidative stress and the postischemic inflammatory response.

Original languageEnglish (US)
Pages (from-to)H2094-H2102
JournalAmerican Journal of Physiology - Heart and Circulatory Physiology
Issue number5 49-5
StatePublished - May 2001


  • Cytokines
  • Energy intake
  • Free radicals
  • Glutathione

ASJC Scopus subject areas

  • Physiology
  • Cardiology and Cardiovascular Medicine
  • Physiology (medical)


Dive into the research topics of 'Calorie restriction attenuates inflammatory responses to myocardial ischemia-reperfusion injury'. Together they form a unique fingerprint.

Cite this