Caloric restriction retards the aging associated changes in γ-aminobutyric acid(A) receptor gene expression in rat cerebellum

Molina C. Mhatre, Maharaj K. Ticku

Research output: Contribution to journalArticlepeer-review

7 Scopus citations

Abstract

It is widely accepted that calorie restriction is an effective way of delaying the aging process. Also, there is an indication that the beneficial effects exerted by dietary manipulation may be due to a direct effect at the molecular level like gene expression. The studies were conducted to determine whether calorie restriction prevents any age-related changes in the structural and molecular aspects of the GABA(A)-BZ receptor. In aged (24-month old diet ad libitum) rats, the binding of [35S]t-butyl-bicyclophosphorothionate (TBPS) was significantly reduced in the cerebellum. In contrast, [35S]TBPS binding remained unchanged in the cerebellum of calorie restricted old rats. In order to evaluate the molecular basis of these changes, the α sub-unit mRNA levels were measured. The GABA(A) receptor α1 sub-unit mRNA level remained unchanged in both the old groups of rats. The α2 subunit mRNA level was significantly decreased in the cerebellum of aged rats (24-month old ad libitum), whereas it remained unchanged in the cerebellum of calorie restricted old animals. These findings indicate a selective age and diet related modulation in the stoichiometry of the GABA(A) receptor in aging.

Original languageEnglish (US)
Pages (from-to)270-275
Number of pages6
JournalMolecular Brain Research
Volume54
Issue number2
DOIs
StatePublished - Mar 1 1998

Keywords

  • α sub-unit
  • Diet
  • GABA(A) receptor
  • mRNA level
  • Radioligand binding

ASJC Scopus subject areas

  • Molecular Biology
  • Cellular and Molecular Neuroscience

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