Calmodulin regulates Ca2+-dependent feedback inhibition of store-operated Ca2+ influx by interaction with a site in the C terminus of TrpC1

Brij B. Singh; Xibao Liu; Jisen Tang; Michael X. Zhu; Indu S. Ambudkar

doi:10.1016/S1097-2765(02)00506-3

Calmodulin regulates Ca²⁺-dependent feedback inhibition of store-operated Ca²⁺ influx by interaction with a site in the C terminus of TrpC1

Brij B. Singh, Xibao Liu, Jisen Tang, Michael X. Zhu, Indu S. Ambudkar

School of Dentistry

Research output: Contribution to journal › Article › peer-review

118 Scopus citations

Abstract

The mechanism involved in [Ca²⁺]_i-dependent feedback inhibition of store-operated Ca²⁺ entry (SOCE) is not yet known. Expression of Ca²⁺-insensitive calmodulin (Mut-CaM) but not wild-type CaM increased SOCE and decreased its Ca²⁺-dependent inactivation. Expression of TrpC1 lacking C terminus aa 664-793 (TrpC1ΔC) also attenuated Ca²⁺-dependent inactivation of SOCE. CaM interacted with endogenous and expressed TrpC1 and with GST-TrpC1 C terminus but not with TrpC1ΔC. Two CaM binding domains, aa 715-749 and aa 758-793, were identified. Expression of TrpC1Δ758-793 but not TrpC1Δ715-749 mimicked the effects of TrpC1ΔC and Mut-CaM on SOCE. These data demonstrate that CaM mediates Ca²⁺-dependent feedback inhibition of SOCE via binding to a domain in the C terminus of TrpC1. These findings reveal an integral role for TrpC1 in the regulation of SOCE.

Original language	English (US)
Pages (from-to)	739-750
Number of pages	12
Journal	Molecular Cell
Volume	9
Issue number	4
DOIs	https://doi.org/10.1016/S1097-2765(02)00506-3
State	Published - 2002

ASJC Scopus subject areas

Molecular Biology
Cell Biology

Access to Document

10.1016/S1097-2765(02)00506-3

Fingerprint Dive into the research topics of 'Calmodulin regulates Ca<sup>2+</sup>-dependent feedback inhibition of store-operated Ca<sup>2+</sup> influx by interaction with a site in the C terminus of TrpC1'. Together they form a unique fingerprint.

Cite this

@article{09811c85211647d0b8878c76380eca66,

title = "Calmodulin regulates Ca2+-dependent feedback inhibition of store-operated Ca2+ influx by interaction with a site in the C terminus of TrpC1",

abstract = "The mechanism involved in [Ca2+]i-dependent feedback inhibition of store-operated Ca2+ entry (SOCE) is not yet known. Expression of Ca2+-insensitive calmodulin (Mut-CaM) but not wild-type CaM increased SOCE and decreased its Ca2+-dependent inactivation. Expression of TrpC1 lacking C terminus aa 664-793 (TrpC1ΔC) also attenuated Ca2+-dependent inactivation of SOCE. CaM interacted with endogenous and expressed TrpC1 and with GST-TrpC1 C terminus but not with TrpC1ΔC. Two CaM binding domains, aa 715-749 and aa 758-793, were identified. Expression of TrpC1Δ758-793 but not TrpC1Δ715-749 mimicked the effects of TrpC1ΔC and Mut-CaM on SOCE. These data demonstrate that CaM mediates Ca2+-dependent feedback inhibition of SOCE via binding to a domain in the C terminus of TrpC1. These findings reveal an integral role for TrpC1 in the regulation of SOCE.",

author = "Singh, {Brij B.} and Xibao Liu and Jisen Tang and Zhu, {Michael X.} and Ambudkar, {Indu S.}",

year = "2002",

doi = "10.1016/S1097-2765(02)00506-3",

language = "English (US)",

volume = "9",

pages = "739--750",

journal = "Molecular Cell",

issn = "1097-2765",

publisher = "Cell Press",

number = "4",

}

TY - JOUR

T1 - Calmodulin regulates Ca2+-dependent feedback inhibition of store-operated Ca2+ influx by interaction with a site in the C terminus of TrpC1

AU - Singh, Brij B.

AU - Liu, Xibao

AU - Tang, Jisen

AU - Zhu, Michael X.

AU - Ambudkar, Indu S.

PY - 2002

Y1 - 2002

N2 - The mechanism involved in [Ca2+]i-dependent feedback inhibition of store-operated Ca2+ entry (SOCE) is not yet known. Expression of Ca2+-insensitive calmodulin (Mut-CaM) but not wild-type CaM increased SOCE and decreased its Ca2+-dependent inactivation. Expression of TrpC1 lacking C terminus aa 664-793 (TrpC1ΔC) also attenuated Ca2+-dependent inactivation of SOCE. CaM interacted with endogenous and expressed TrpC1 and with GST-TrpC1 C terminus but not with TrpC1ΔC. Two CaM binding domains, aa 715-749 and aa 758-793, were identified. Expression of TrpC1Δ758-793 but not TrpC1Δ715-749 mimicked the effects of TrpC1ΔC and Mut-CaM on SOCE. These data demonstrate that CaM mediates Ca2+-dependent feedback inhibition of SOCE via binding to a domain in the C terminus of TrpC1. These findings reveal an integral role for TrpC1 in the regulation of SOCE.

AB - The mechanism involved in [Ca2+]i-dependent feedback inhibition of store-operated Ca2+ entry (SOCE) is not yet known. Expression of Ca2+-insensitive calmodulin (Mut-CaM) but not wild-type CaM increased SOCE and decreased its Ca2+-dependent inactivation. Expression of TrpC1 lacking C terminus aa 664-793 (TrpC1ΔC) also attenuated Ca2+-dependent inactivation of SOCE. CaM interacted with endogenous and expressed TrpC1 and with GST-TrpC1 C terminus but not with TrpC1ΔC. Two CaM binding domains, aa 715-749 and aa 758-793, were identified. Expression of TrpC1Δ758-793 but not TrpC1Δ715-749 mimicked the effects of TrpC1ΔC and Mut-CaM on SOCE. These data demonstrate that CaM mediates Ca2+-dependent feedback inhibition of SOCE via binding to a domain in the C terminus of TrpC1. These findings reveal an integral role for TrpC1 in the regulation of SOCE.

UR - http://www.scopus.com/inward/record.url?scp=0036238275&partnerID=8YFLogxK

UR - http://www.scopus.com/inward/citedby.url?scp=0036238275&partnerID=8YFLogxK

U2 - 10.1016/S1097-2765(02)00506-3

DO - 10.1016/S1097-2765(02)00506-3

M3 - Article

C2 - 11983166

AN - SCOPUS:0036238275

VL - 9

SP - 739

EP - 750

JO - Molecular Cell

JF - Molecular Cell

SN - 1097-2765

IS - 4

ER -