Calcium Calmodulin Dependent Phosphorylation of Proteins: Fetal Cortical Neurons and Adult Cortex

Haviryaji S.G. Kalluri, Maharaj K. Ticku

Research output: Contribution to journalArticlepeer-review

Abstract

In the present investigation, changes in the calcium calmodulin-dependent phosphorylation of proteins have been examined in murine fetal cortical neurons and adult cortex. An ∼80-kD protein in the fetal neurons was not phosphorylated/dephosphorylated in a calmodulin-dependent manner. However, this protein was phosphorylated by PMA both in the presence and absence of calcium. These data suggest that calmodulin inhibits the phosphorylation of a ∼80-kD protein by inhibiting PKC in murine fetal cortical neurons but not in the adult cortex. More importantly, we demonstrate that the calmodulin-mediated inhibition of phosphorylation was restored by preincubating the cortical neurons with KN-62, a CaM kinase inhibitor.

Original languageEnglish (US)
Pages (from-to)781-784
Number of pages4
JournalNeurochemical Research
Volume29
Issue number4
DOIs
StatePublished - Apr 2004

Keywords

  • Autophosphorylation
  • CaM kinase II
  • Development

ASJC Scopus subject areas

  • Biochemistry
  • Cellular and Molecular Neuroscience

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