C3 reacted Sepharose: A preparative method for separating T and B lymphocytes

P. Casali, B. M. Perussia

Research output: Contribution to journalArticle

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Abstract

Lymphocytes bearing complement receptors were separated from lymphocyte suspensions using C3-reacted Sepharose columns as solid phase immunoabsorbents. The retained CR+lymphocytes were recovered by elution of the column with IgG fraction purified from a rabbit antiserum to human C3. This paper describes the method, its specificity and the yields of cells obtained.

Original languageEnglish (US)
Pages (from-to)38-42
Number of pages5
JournalClinical and Experimental Immunology
Volume27
Issue number1
StatePublished - Dec 1 1977
Externally publishedYes

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ASJC Scopus subject areas

  • Immunology and Allergy
  • Immunology

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