c-fos and HSP70 gene expression in rat brains in high gravitation-induced cerebral ischemia

Marjatta Son, Asha R. Shahed, Paul M. Werchan, John C. Lee

Research output: Contribution to journalArticle

4 Scopus citations

Abstract

Previous studies have shown that brief exposures of rodents to high gravitation forces (+GZ) in a specifically designed centrifuge cause global cerebral ischemia. In the present study, the effect of +GZ exposure to +22.5GZ for 15 to 60 s on c-fos and HSP70 gene expression was examined. Northern and RT-PCR analyses of total RNA isolated from brains of rats at different post-exposure times revealed a significant, time-dependent increase in the c-fos mRNA level which returned to near normal by 180 min. The HSP70 mRNA level was increased two-fold at 30 min post exposure, and remained elevated until 180 min. The transient stimulation of c-fos and HSP70 gene expression should serve as useful biomarkers for hypergravic stress on the brain. The present results should aid in design of future experiments in our understanding of the pathophysiology of the high +GZ challenges.

Original languageEnglish (US)
Pages (from-to)81-84
Number of pages4
JournalNeuroscience Letters
Volume200
Issue number2
DOIs
StatePublished - Nov 17 1995

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Keywords

  • Cerebral ischemia
  • Gene expression
  • Gravitation force
  • HSP70
  • Loss of consciousness
  • c-fos

ASJC Scopus subject areas

  • Neuroscience(all)

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