TY - JOUR
T1 - Burn induces a Th-17 inflammatory response at the injury site
AU - Sasaki, Jennifer R.
AU - Zhang, Qiong
AU - Schwacha, Martin G.
N1 - Funding Information:
These studies were supported by funding from NIH grant GM79122 and were presented in part at the 42nd annual meeting of the American Burn Association in Boston, Massachusetts. Ms. Sasaki conducted these studies while participating in the UTHSCSA Medical Student Research Program.
PY - 2011/6
Y1 - 2011/6
N2 - Introduction: The high incidence of morbidity and mortality following major burn can be in part attributed to immune dysfunction and wound healing complications. Inflammation plays a major role in the complex process of wound repair. Recently, a novel class of T-helper cells, termed Th-17 cells, has been found to secrete the pro-inflammatory cytokines IL-17 and IL-22. The Th-17 response also involves other cytokines, such as IL-6 and TGF-β, which have been shown to be associated with burn-induced inflammation. Nonetheless, the relationships between the Th-17 response and post-burn inflammation are unknown. Methods: C57BL/6 male mice (n = 5-6/group) were subjected to a major burn (25% TBSA) or sham procedure. Three hours thereafter, skin samples were collected (uninjured skin and burn skin) and processed for the determination of Th-17 cytokine (IL-6, IL-17, IL-22, IL-23, IL-27, and TGF-β) levels by ELISA. Results: At 3 h after burn a significant (∼3-fold) increase in tissue levels of IL-17 and IL-22 was observed at the burn site as compared to sham skin. The burn-induced Th-17 response was independent of statistically significant changes in other Th-17 cytokines (i.e., IL-6, IL-23, IL-27 and TGF-β). Conclusions: These findings indicate the development of a robust Th-17 response at the burn site that may play an important role in subsequent immune and wound healing derangements.
AB - Introduction: The high incidence of morbidity and mortality following major burn can be in part attributed to immune dysfunction and wound healing complications. Inflammation plays a major role in the complex process of wound repair. Recently, a novel class of T-helper cells, termed Th-17 cells, has been found to secrete the pro-inflammatory cytokines IL-17 and IL-22. The Th-17 response also involves other cytokines, such as IL-6 and TGF-β, which have been shown to be associated with burn-induced inflammation. Nonetheless, the relationships between the Th-17 response and post-burn inflammation are unknown. Methods: C57BL/6 male mice (n = 5-6/group) were subjected to a major burn (25% TBSA) or sham procedure. Three hours thereafter, skin samples were collected (uninjured skin and burn skin) and processed for the determination of Th-17 cytokine (IL-6, IL-17, IL-22, IL-23, IL-27, and TGF-β) levels by ELISA. Results: At 3 h after burn a significant (∼3-fold) increase in tissue levels of IL-17 and IL-22 was observed at the burn site as compared to sham skin. The burn-induced Th-17 response was independent of statistically significant changes in other Th-17 cytokines (i.e., IL-6, IL-23, IL-27 and TGF-β). Conclusions: These findings indicate the development of a robust Th-17 response at the burn site that may play an important role in subsequent immune and wound healing derangements.
KW - Cytokines
KW - Immune dysfunction
KW - Skin
KW - T-cells
KW - Wound healing
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U2 - 10.1016/j.burns.2011.01.028
DO - 10.1016/j.burns.2011.01.028
M3 - Article
C2 - 21353393
AN - SCOPUS:79955051759
VL - 37
SP - 646
EP - 651
JO - Burns
JF - Burns
SN - 0305-4179
IS - 4
ER -