Burn-induced alterations in toll-like receptor-mediated responses by bronchoalveolar lavage cells

Richard F. Oppeltz, Meenakshi Rani, Qiong Zhang, Martin G Schwacha

Research output: Contribution to journalArticle

19 Citations (Scopus)

Abstract

Burn is associated with profound inflammation and activation of the innate immune system in multiple organ beds, including the lung. Similarly, toll-like receptors (TLR) are associated with innate immune activation. Nonetheless, it is unclear what impact burn has on TLR-induced inflammatory responses in the lung. Methods: Male C57BL/6 mice were subjected to burn (3rd degree, 25% TBSA) or sham procedure and 1, 3 or 7. days thereafter, bronchoalveolar lavage (BAL) fluid was collected and cells were isolated and cultured in vitro with specific TLR agonists as follows: Zymosan (TLR-2), LPS (TLR-4) and CpG-ODN (TLR-9). Supernatants were collected 48. h later and assayed for inflammatory cytokine levels (IL-1β, IL-6, IL-10, IL-17, TNF-α, KC, MCP-1, MIP-1α, MIP-1β and RANTES) by Bioplex. Results: BAL fluid from sham and burn mice did not contain detectable cytokine levels. BAL cells, irrespective of injury, were responsive to TLR-2 and TLR-4 activation. Seven days after burn, TLR-2 and TLR-4 mediated responses by BAL cells were enhanced as evidenced by increased production of IL-6, IL-17, TNF-α, MCP-1, MIP-1β and RANTES. Conclusions: Burn-induced changes in TLR-2 and TLR-4 reactivity may contribute to the development of post-burn complications, such as acute lung injury (ALI) and adult respiratory distress syndrome (ARDS).

Original languageEnglish (US)
Pages (from-to)396-401
Number of pages6
JournalCytokine
Volume55
Issue number3
DOIs
StatePublished - Sep 2011

Fingerprint

Toll-Like Receptor 2
Toll-Like Receptor 4
Toll-Like Receptors
Bronchoalveolar Lavage
Burns
Chemokine CCL5
Interleukin-17
Chemical activation
Bronchoalveolar Lavage Fluid
Interleukin-6
Toll-Like Receptor 9
Cytokines
Lung
Zymosan
Fluids
Acute Lung Injury
Immune system
Adult Respiratory Distress Syndrome
Interleukin-1
Inbred C57BL Mouse

Keywords

  • Burn
  • Cytokines
  • Lung

ASJC Scopus subject areas

  • Immunology
  • Immunology and Allergy
  • Hematology
  • Biochemistry
  • Molecular Biology

Cite this

Burn-induced alterations in toll-like receptor-mediated responses by bronchoalveolar lavage cells. / Oppeltz, Richard F.; Rani, Meenakshi; Zhang, Qiong; Schwacha, Martin G.

In: Cytokine, Vol. 55, No. 3, 09.2011, p. 396-401.

Research output: Contribution to journalArticle

Oppeltz, Richard F. ; Rani, Meenakshi ; Zhang, Qiong ; Schwacha, Martin G. / Burn-induced alterations in toll-like receptor-mediated responses by bronchoalveolar lavage cells. In: Cytokine. 2011 ; Vol. 55, No. 3. pp. 396-401.
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