Burn enhances toll-like receptor induced responses by circulating leukocytes

Martin G. Schwacha, Qiong Zhang, Meenakshi Rani, Teresa Craig, Richard F. Oppeltz

Research output: Contribution to journalArticlepeer-review

13 Scopus citations

Abstract

Burn and toll-like receptors (TLR) are associated with innate immune system activation, but the impact of burn on TLR-induced inflammation responses by circulating leukocytes is unknown. To study this, C57BL/6 mice were subjected to burn (3 rd degree, 25% TBSA) or sham procedure and 1-7 days later blood was collected. Whole blood cell suspensions were incubated for 24 hr in the presence of zymosan (TLR-2 agonist) or LPS (TLR-4 agonist). The blood cultures were responsive to TLR2 and TLR4-mediated activation, resulting in the production of IL-6, IL-10, IL-17, TNF-α, MIP-1α, MIP-1β, KC and RANTES. TLR2-induced KC and MIP-1β production was greater in the burn group at 3-7 days post-injury, whereas IL-6, IL-10, KC and MIP-1β were greater for TLR4-induced activation after burn. In conclusion, circulating leukocytes were responsive to TLR-induced activation and TLR-mediated inflammatory responses were enhanced 3-7 days post-injury, as evidenced by increased production of these inflammatory mediators.

Original languageEnglish (US)
Pages (from-to)136-144
Number of pages9
JournalInternational Journal of Clinical and Experimental Medicine
Volume5
Issue number2
StatePublished - Apr 26 2012

Keywords

  • Blood
  • Burn
  • Cytokines
  • Inflammation
  • Toll-like receptors

ASJC Scopus subject areas

  • Biochemistry, Genetics and Molecular Biology(all)

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