BRG1 Activates PR65A Transcription to Regulate NO Bioavailability in Vascular Endothelial Cells

Baoyu Chen, Qianwen Zhao, Tongchang Xu, Liming Yu, Lili Zhuo, Yuyu Yang, Yong Xu

Research output: Contribution to journalArticlepeer-review

18 Scopus citations

Abstract

Vascular endothelial cells contribute to the pathogenesis of cardiovascular diseases by producing and disseminating angiocrine factors. Nitric oxide (NO), catalyzed by endothelial NO synthase (eNOS), is one of the prototypical angiocrine factors. eNOS activity is modulated by site-specific phosphorylation. We have previously shown that endothelial-specific knockdown of BRG1 in Apoe–/– mice attenuates the development of atherosclerosis, in which eNOS-dependent NO catalysis plays an antagonizing role. Here we report that attenuation of atherogenesis in mice by BRG1 knockdown was accompanied by partial restoration of NO biosynthesis by 44% in the arteries and a simultaneous up-regulation of eNOS serine 1177 phosphorylation by 59%. Indeed, BRG1 depletion or inhibition ameliorated oxLDL-induced loss of NO bioavailability and eNOS phosphorylation in cultured endothelial cells. Further analysis revealed that BRG1 regulated eNOS phosphorylation and NO synthesis by activating the transcription of protein phosphatase 2A (PP2A) structural subunit a (encoded by PR65A). BRG1 interacted with ETS1, was recruited by ETS1 to the PR65A promoter, and cooperated with ETS1 to activate PR65A transcription. Finally, depletion of ETS1, similar to BRG1, repressed PR65A induction, normalized eNOS phosphorylation, and rescued NO biosynthesis in endothelial cells treated with oxLDL. In conclusion, our data characterize a novel transcriptional cascade that regulates NO bioavailability in vascular endothelial cells.

Original languageEnglish (US)
Article number774
JournalFrontiers in Cell and Developmental Biology
Volume8
DOIs
StatePublished - Aug 12 2020
Externally publishedYes

Keywords

  • BRG1
  • eNOS
  • nitric oxide
  • phosphatase
  • phosphorylation
  • PP2A
  • transcriptional regulation
  • vascular endothelial cells

ASJC Scopus subject areas

  • Developmental Biology
  • Cell Biology

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