To determine the metabolic basis of dietary programming of HDL concentrations by infant diet we measured serum lipid transfer activities at 97 weeks of age in 35 baboons. Breast-fed and formulafed baboons were fed a high cholesterol, saturated fat diet after weaning at 15 weeks, cholesterol ester transfer protein (CETP), phospholipid transfer protein (PLTP), and lecithin cholesterol acyl-transferase (LCAT) were measured using exogenous substrates and HDL subfractions were isolated by ultracentrifugation. Among breast-fed baboons CETP activity at 97 weeks was 16% higher (P < 0.075) and LCAT was 10% lower (P < 0.09) compared with those fed formula. PLTP activity did not differ by infant diet. HDLl-cholesterol (C) was 22% lower, HDL2-C 13% lower, and HDL3-C 17% lower among breast-fed compared with formula-fed baboons. HDL1-C and HDL3-C were negatively correlated with CETP activity fr = -0.33, P = 0.08 and r = -0.32. P = 0.09 respectively). We conclude that postweaning differences in CETP and LCAT activities may mediate the differences in HDL-C fractions of breast- vs formula-fed baboons. Supported by the American Heart Association, Texas Affiliate grant #94G-356, and NIH Grant ROI HD 24308.
|Original language||English (US)|
|State||Published - Dec 1 1996|
ASJC Scopus subject areas
- Molecular Biology