Break-induced replication: unraveling each step

Liping Liu; Anna Malkova

doi:10.1016/j.tig.2022.03.011

Break-induced replication: unraveling each step

Liping Liu, Anna Malkova

Research output: Contribution to journal › Review article › peer-review

32 Scopus citations

Abstract

Break-induced replication (BIR) repairs one-ended double-strand DNA breaks through invasion into a homologous template followed by DNA synthesis. Different from S-phase replication, BIR copies the template DNA in a migrating displacement loop (D-loop) and results in conservative inheritance of newly synthesized DNA. This unusual mode of DNA synthesis makes BIR a source of various genetic instabilities like those associated with cancer in humans. This review focuses on recent progress in delineating the mechanism of Rad51-dependent BIR in budding yeast. In addition, we discuss new data that describe changes in BIR efficiency and fidelity on encountering replication obstacles as well as the implications of these findings for BIR-dependent processes such as telomere maintenance and the repair of collapsed replication forks.

Original language	English (US)
Pages (from-to)	752-765
Number of pages	14
Journal	Trends in Genetics
Volume	38
Issue number	7
DOIs	https://doi.org/10.1016/j.tig.2022.03.011
State	Published - Jul 2022
Externally published	Yes

Keywords

break-induced replication (BIR)
kinetics and rate of BIR
lagging-strand BIR synthesis
replication obstacles
yeast

ASJC Scopus subject areas

Genetics

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10.1016/j.tig.2022.03.011

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title = "Break-induced replication: unraveling each step",

abstract = "Break-induced replication (BIR) repairs one-ended double-strand DNA breaks through invasion into a homologous template followed by DNA synthesis. Different from S-phase replication, BIR copies the template DNA in a migrating displacement loop (D-loop) and results in conservative inheritance of newly synthesized DNA. This unusual mode of DNA synthesis makes BIR a source of various genetic instabilities like those associated with cancer in humans. This review focuses on recent progress in delineating the mechanism of Rad51-dependent BIR in budding yeast. In addition, we discuss new data that describe changes in BIR efficiency and fidelity on encountering replication obstacles as well as the implications of these findings for BIR-dependent processes such as telomere maintenance and the repair of collapsed replication forks.",

keywords = "break-induced replication (BIR), kinetics and rate of BIR, lagging-strand BIR synthesis, replication obstacles, yeast",

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AU - Malkova, Anna

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AB - Break-induced replication (BIR) repairs one-ended double-strand DNA breaks through invasion into a homologous template followed by DNA synthesis. Different from S-phase replication, BIR copies the template DNA in a migrating displacement loop (D-loop) and results in conservative inheritance of newly synthesized DNA. This unusual mode of DNA synthesis makes BIR a source of various genetic instabilities like those associated with cancer in humans. This review focuses on recent progress in delineating the mechanism of Rad51-dependent BIR in budding yeast. In addition, we discuss new data that describe changes in BIR efficiency and fidelity on encountering replication obstacles as well as the implications of these findings for BIR-dependent processes such as telomere maintenance and the repair of collapsed replication forks.

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KW - replication obstacles

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Break-induced replication: unraveling each step

Abstract

Keywords

ASJC Scopus subject areas

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