BRCA1 modulates ionizing radiation-induced nuclear focus formation by the replication protein A p34 subunit

Shailesh K. Choudhary, Rong Li

Research output: Contribution to journalArticlepeer-review

33 Scopus citations

Abstract

Mutations in BRCA1 account for a significant proportion of familial breast and ovarian cancers. BRCA1 has been implicated in DNA damage responses including double-strand break (DSB) repair. However, its exact role in DSB repair and its functional relationship with other known repair proteins remain to be elucidate. In this study, we carried out a cytological analysis of the effect of BRCA1 on damage-induced nuclear focus formation mediated by the replication protein A (RPA). RPA is a multi-functional protein that participates in both DNA replication and various types of DNA repair including DSB repair. Following ionizing radiation (IR), RPA and BRCA1 formed punctate nuclear staining patterns that co-localized with each other, consistent with the implicated roles of both proteins in the same repair process. The number of damage-induced RPA foci in BRCA1-deficient cells, however, was significantly greater than that in BRCA1-positive cells. Moreover, the effect of BRCA1 on the RPA staining pattern appeared to be specific for IR but not ultraviolet (UV) irradiation. These data suggest that BRCA1 plays an important role in processing the RPA-associated intermediates during DSB repair.

Original languageEnglish (US)
Pages (from-to)666-674
Number of pages9
JournalJournal of Cellular Biochemistry
Volume84
Issue number4
DOIs
StatePublished - Feb 11 2002

Keywords

  • BRCA1
  • Double strand break repair
  • Ionizing radiation
  • Nuclear staining
  • Replication protein A

ASJC Scopus subject areas

  • Biochemistry
  • Molecular Biology
  • Cell Biology

Fingerprint Dive into the research topics of 'BRCA1 modulates ionizing radiation-induced nuclear focus formation by the replication protein A p34 subunit'. Together they form a unique fingerprint.

Cite this