Branch Migration Activity of Rad54 Protein

Olga M. Mazina, Alexander V. Mazin

Research output: Chapter in Book/Report/Conference proceedingChapter


Rad54 is a eukaryotic protein that plays an important role in homologous recombination. Rad54, a member of the Swi2/Snf2 family, binds to Holliday junctions with high specificity and promotes their branch migration in an ATP hydrolysis-dependent manner. Here we describe the methods our laboratory used to characterize the branch migration activity of Rad54. These assays are applicable for other branch migration proteins regardless of whether they have canonical helicase activity or not.

Original languageEnglish (US)
Title of host publicationMethods in Molecular Biology
PublisherHumana Press
Number of pages23
StatePublished - 2021
Externally publishedYes

Publication series

NameMethods in Molecular Biology
ISSN (Print)1064-3745
ISSN (Electronic)1940-6029


  • Branch migration
  • Holliday junctions
  • Homologous recombination
  • Rad54 protein

ASJC Scopus subject areas

  • Genetics
  • Molecular Biology


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