Bone to total alkaline phosphatase ratios improve sensitivity and specificity of bone alkaline phosphatase immunoassays

Lori J. Sokoll, Martin H. Kroll, Michael A. Levine, F. Fred Poordad, Daniel W. Chan

Research output: Contribution to journalArticle

2 Scopus citations

Abstract

Objectives: Evaluate the ability of two bone alkaline phosphatase (ALPs) immunoassays (Ostase, Hybritech Inc and Alkphase-B, Metra Biosystems) to clinically differentiate between osseous and non-osseous ALP sources. Design and Methods: Specimens from patients with either liver or bone disease (Paget's disease or metastatic cancer) were analyzed by both methods. Results: There was a good correlation between these two assays. Values for ALP(B), whether determined as a concentration by the Ostase assay or as an activity by the Alkphase-B assay, were similar for subjects with liver disease or bone disease. However, total ALP (ALP(T)) activity was higher in liver disease compared to bone. When ALP(B) was expressed in relation to ALP(T), ratios were significantly greater in subjects with bone disease than in those with liver disease. ALP(B)/ALP(T) ratios improved the specificity of the Ostase assay from 52% to 86% and the Alkphase-B assay from 58% to 74%. Conclusions: These two ALPs assays have good analytical performance and their clinical utility can be enhanced by expressing ALP(B) values in relation to ALP(T) activity.

Original languageEnglish (US)
Pages (from-to)625-629
Number of pages5
JournalClinical Biochemistry
Volume30
Issue number8
DOIs
StatePublished - Dec 1 1997
Externally publishedYes

Keywords

  • Bone metabolism
  • Immunoassay
  • Isoenzymes

ASJC Scopus subject areas

  • Clinical Biochemistry

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