TY - JOUR
T1 - Bone tissue engineering using human mesenchymal stem cells
T2 - Effects of scaffold material and medium flow
AU - Meinel, Lorenz
AU - Karageorgiou, Vassilis
AU - Fajardo, Robert
AU - Snyder, Brian
AU - Shinde-Patil, Vivek
AU - Zichner, Ludwig
AU - Kaplan, David
AU - Langer, Robert
AU - Vunjak-Novakovic, Gordana
N1 - Funding Information:
This work was supported by the National Aeronautics and Space Administration, National Institutes of Health, and the Alexander von Humboldt Foundation (Feodor-Lynen fellowship to LM). The authors thank Wyeth Bio-pharmaceuticals for generously providing BMP-2, Sandra Hofmann for technical assistance with pellet cultures, and Sue Kangiser for help with the manuscript preparation.
Copyright:
Copyright 2012 Elsevier B.V., All rights reserved.
PY - 2004/1
Y1 - 2004/1
N2 - We report studies of bone tissue engineering using human mesenchymal stem cells (MSCs), a protein substrate (film or scaffold; fast degrading unmodified collagen, or slowly degrading cross-linked collagen and silk), and a bioreactor (static culture, spinner flask, or perfused cartridge). MSCs were isolated from human bone marrow, characterized for the expression of cell surface markers and the ability to undergo chondrogenesis and osteogenesis in vitro, and cultured for 5 weeks. MSCs were positive for CD105/endoglin, and had a potential for chondrogenic and osteogenic differentiation. In static culture, calcium deposition was similar for MSC grown on collagen scaffolds and films. Under medium flow, MSC on collagen scaffolds deposited more calcium and had a higher alcaline phosphatase (AP) activity than MSC on collagen films. The amounts of DNA were markedly higher in constructs based on slowly degrading (modified collagen and silk) scaffolds than on fast degrading (unmodified collagen) scaffolds. In spinner flasks, medium flow around constructs resulted in the formation of bone rods within the peripheral region, that were interconnected and perpendicular to the construct surface, whereas in perfused constructs, individual bone rods oriented in the direction of fluid flow formed throughout the construct volume. These results suggest that osteogenesis in cultured MSC can be modulated by scaffold properties and flow environment.
AB - We report studies of bone tissue engineering using human mesenchymal stem cells (MSCs), a protein substrate (film or scaffold; fast degrading unmodified collagen, or slowly degrading cross-linked collagen and silk), and a bioreactor (static culture, spinner flask, or perfused cartridge). MSCs were isolated from human bone marrow, characterized for the expression of cell surface markers and the ability to undergo chondrogenesis and osteogenesis in vitro, and cultured for 5 weeks. MSCs were positive for CD105/endoglin, and had a potential for chondrogenic and osteogenic differentiation. In static culture, calcium deposition was similar for MSC grown on collagen scaffolds and films. Under medium flow, MSC on collagen scaffolds deposited more calcium and had a higher alcaline phosphatase (AP) activity than MSC on collagen films. The amounts of DNA were markedly higher in constructs based on slowly degrading (modified collagen and silk) scaffolds than on fast degrading (unmodified collagen) scaffolds. In spinner flasks, medium flow around constructs resulted in the formation of bone rods within the peripheral region, that were interconnected and perpendicular to the construct surface, whereas in perfused constructs, individual bone rods oriented in the direction of fluid flow formed throughout the construct volume. These results suggest that osteogenesis in cultured MSC can be modulated by scaffold properties and flow environment.
KW - Bioreactor
KW - Collagen
KW - Flow
KW - Scaffold
KW - Silk
KW - μ-Computerized Tomography
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U2 - 10.1023/B:ABME.0000007796.48329.b4
DO - 10.1023/B:ABME.0000007796.48329.b4
M3 - Article
C2 - 14964727
AN - SCOPUS:2942588974
VL - 32
SP - 112
EP - 122
JO - Annals of Biomedical Engineering
JF - Annals of Biomedical Engineering
SN - 0090-6964
IS - 1
ER -