Bone resorption caused by three periodontal pathogens in vivo in mice is mediated in part by prostaglandin

Yuval Zubery, Colin R. Dunstan, Beryl M. Story, Akshmyya Kesavalu, Jeffrey L. Ebersole, Stanley C. Holt, Brendan F. Boyce

Research output: Contribution to journalArticle

61 Scopus citations

Abstract

Gingival inflammation, bacterial infection, alveolar bone destruction, and subsequent tooth loss are characteristic features of periodontal disease, but the precise mechanisms of bone loss are poorly understood. Most animal models of the disease require injury to gingival tissues or teeth, and the effects of microorganisms are thus complicated by host responses to tissue destruction. To determine whether three putative periodontal pathogens, Porphyromonas gingivalis, Campylobacter rectus, and Fusobacterium nucleatum, could cause localized bone resorption in vivo in the absence of tissue injury, we injected live or heat-killed preparations of these microorganisms into the subcutaneous tissues overlying the calvaria of normal mice once daily for 6 days and then examined the bones histologically. We found that all three microorganisms (both live and heat killed) stimulated bone resorption and that the strain of F. nucleatum used appeared to be the strongest inducer of osteoclast activity. Treatment of the mice concomitantly with indomethacin reduced but did not completely inhibit bone resorption by these microorganisms, suggesting that their effects were mediated, in part, by arachidonic acid metabolites (e.g., prostaglandins). Our findings indicate that these potential pathogens can stimulate bone resorption locally when placed beside a bone surface in vivo in the absence of prior tissue injury and support a role for them in the pathogenesis of hone loss around teeth in periodontitis.

Original languageEnglish (US)
Pages (from-to)4158-4162
Number of pages5
JournalInfection and immunity
Volume66
Issue number9
DOIs
StatePublished - 1998

ASJC Scopus subject areas

  • Parasitology
  • Microbiology
  • Immunology
  • Infectious Diseases

Fingerprint Dive into the research topics of 'Bone resorption caused by three periodontal pathogens in vivo in mice is mediated in part by prostaglandin'. Together they form a unique fingerprint.

  • Cite this