Bone morphogenetic protein 2 transcripts in rapidly developing deer antler tissue contain an extended 5' non-coding region arising from a distal promoter

Jian Q. Feng, Di Chen, Nandini Ghosh-Choudhury, Javier Esparza, Gregory R. Mundy, Stephen E. Harris

Research output: Contribution to journalArticlepeer-review

35 Scopus citations

Abstract

To understand the regulation of the BMP-2 gene expression, we recently isolated the BMP-2 gene from a mouse genomic library and characterized the exon-intron structure and promoter. RNase protection assay using poly (A)+ RNA of mouse osteoblasts demonstrates that two regions in BMP-2 gene are protected by antisense mouse BMP-2 RNA probes. These results demonstrate that BMP-2 gene utilizes two alternative promoters, a distal and a proximal promoter. In the present study we demonstrate that BMP-2 mRNA from rapidly growing deer antler tissue has an extended 5' non-coding region compared with the human and rat BMP-2 mRNA. The extended 5' non-coding region in the deer mRNA represents transcripts from the upstream distal promoter. This is the first evidence of a natural BMP-2 mRNA from a bone-forming tissue that most likely initiated from the distal transcription start site.

Original languageEnglish (US)
Pages (from-to)47-52
Number of pages6
JournalBiochimica et Biophysica Acta - Gene Structure and Expression
Volume1350
Issue number1
DOIs
StatePublished - Jan 3 1997

Keywords

  • 5' non-coding region
  • alternative promoter
  • bone morphogenetic protein 2
  • deer antler

ASJC Scopus subject areas

  • Structural Biology
  • Biophysics
  • Biochemistry
  • Genetics

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