BOLD fMRI of visual and somatosensory-motor stimulations in baboons

Hsiao Ying Wey, Jinqi Li, Charles A Szabo, Peter T Fox, M. Michelle Leland, Lisa Jones, Timothy Q. Duong

Research output: Contribution to journalArticle

11 Citations (Scopus)

Abstract

Baboon, with its large brain size and extensive cortical folding compared to other non-human primates, serves as a good model for neuroscience research. This study reports the implementation of a baboon model for blood oxygenation level-dependent (BOLD) fMRI studies (1.5×1.5×4 mm resolution) on a clinical 3T-MRI scanner. BOLD fMRI responses to hypercapnic (5% CO2) challenge, 10Hz flicker visual, and vibrotactile somatosensory-motor stimulations were investigated in baboons anesthetized sequentially with isoflurane and ketamine. Hypercapnia evoked robust BOLD increases. Paralysis was determined to be necessary to achieve reproducible functional activations within and between subjects under our experimental conditions. With optimized anesthetic doses (0.8-1.0% isoflurane or 6-8mg/kg/h ketamine) and adequate paralysis (vecuronium, 0.2mg/kg), robust activations were detected in the visual (V), primary (S1) and secondary (S2) somatosensory, primary motor (M cortices), supplementary motor area (SMA), lateral geniculate nucleus (LGN) and thalamus (Th). Data were tabulated for 11 trials under isoflurane and 10 trials under ketamine on 5 baboons. S1, S2, M, and V activations were detected in essentially all trials (90-100% of the time, except 82% for S2 under isoflurane and 70% for M under ketamine). LGN activations were detected 64-70% of the time under both anesthetics. SMA and Th activations were detected 36-45% of the time under isoflurane and 60% of the time under ketamine. BOLD percent changes among different structures were slightly higher under ketamine than isoflurane (0.75% versus 0.58% averaging all structures), but none was statistically different (P>0.05). This baboon model offers an opportunity to non-invasively image brain functions and dysfunctions in large non-human primates.

Original languageEnglish (US)
Pages (from-to)1420-1427
Number of pages8
JournalNeuroImage
Volume52
Issue number4
DOIs
StatePublished - Oct 2010

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Papio
Isoflurane
Ketamine
Magnetic Resonance Imaging
Motor Cortex
Geniculate Bodies
Paralysis
Primates
Anesthetics
Lateral Thalamic Nuclei
Vecuronium Bromide
Hypercapnia
Brain
Neurosciences
Thalamus
Research

Keywords

  • Anesthetic
  • Animal models
  • Blood oxygenation level-dependent (BOLD) fMRI
  • Brain mapping
  • Cerebral blood flow
  • Non-human primates
  • Thalamus

ASJC Scopus subject areas

  • Cognitive Neuroscience
  • Neurology

Cite this

Wey, H. Y., Li, J., Szabo, C. A., Fox, P. T., Leland, M. M., Jones, L., & Duong, T. Q. (2010). BOLD fMRI of visual and somatosensory-motor stimulations in baboons. NeuroImage, 52(4), 1420-1427. https://doi.org/10.1016/j.neuroimage.2010.05.014

BOLD fMRI of visual and somatosensory-motor stimulations in baboons. / Wey, Hsiao Ying; Li, Jinqi; Szabo, Charles A; Fox, Peter T; Leland, M. Michelle; Jones, Lisa; Duong, Timothy Q.

In: NeuroImage, Vol. 52, No. 4, 10.2010, p. 1420-1427.

Research output: Contribution to journalArticle

Wey, HY, Li, J, Szabo, CA, Fox, PT, Leland, MM, Jones, L & Duong, TQ 2010, 'BOLD fMRI of visual and somatosensory-motor stimulations in baboons', NeuroImage, vol. 52, no. 4, pp. 1420-1427. https://doi.org/10.1016/j.neuroimage.2010.05.014
Wey, Hsiao Ying ; Li, Jinqi ; Szabo, Charles A ; Fox, Peter T ; Leland, M. Michelle ; Jones, Lisa ; Duong, Timothy Q. / BOLD fMRI of visual and somatosensory-motor stimulations in baboons. In: NeuroImage. 2010 ; Vol. 52, No. 4. pp. 1420-1427.
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