TY - JOUR
T1 - Bmp2 deletion causes an amelogenesis imperfecta phenotype via regulating enamel gene expression
AU - Guo, Feng
AU - Feng, Junsheng
AU - Wang, Feng
AU - Li, Wentong
AU - Gao, Qingping
AU - Chen, Zhuo
AU - Shoff, Lisa
AU - Donly, Kevin J.
AU - Gluhak-Heinrich, Jelica
AU - Chun, Yong Hee Patricia
AU - Harris, Stephen E.
AU - Macdougall, Mary
AU - Chen, Shuo
N1 - Publisher Copyright:
© 2015 Wiley Periodicals, Inc.
PY - 2015/8/1
Y1 - 2015/8/1
N2 - Although Bmp2 is essential for tooth formation, the role of Bmp2 during enamel formation remains unknown in vivo. In this study, the role of Bmp2 in regulation of enamel formation was investigated by the Bmp2 conditional knock out (Bmp2 cKO) mice. Teeth of Bmp2 cKO mice displayed severe and profound phenotypes with asymmetric and misshaped incisors as well as abrasion of incisors and molars. Scanning electron microscopy analysis showed that the enamel layer was hypoplastic and enamel lacked a typical prismatic pattern. Teeth from null mice were much more brittle as tested by shear and compressive moduli. Expression of enamel matrix protein genes, amelogenin, enamelin, and enamel-processing proteases, Mmp-20 and Klk4 was reduced in the Bmp2 cKO teeth as reflected in a reduced enamel formation. Exogenous Bmp2 up-regulated those gene expressions in mouse enamel organ epithelial cells. This result for the first time indicates Bmp2 signaling is essential for proper enamel development and mineralization in vivo. J. Cell. Physiol. 230: 1871-1882, 2015.
AB - Although Bmp2 is essential for tooth formation, the role of Bmp2 during enamel formation remains unknown in vivo. In this study, the role of Bmp2 in regulation of enamel formation was investigated by the Bmp2 conditional knock out (Bmp2 cKO) mice. Teeth of Bmp2 cKO mice displayed severe and profound phenotypes with asymmetric and misshaped incisors as well as abrasion of incisors and molars. Scanning electron microscopy analysis showed that the enamel layer was hypoplastic and enamel lacked a typical prismatic pattern. Teeth from null mice were much more brittle as tested by shear and compressive moduli. Expression of enamel matrix protein genes, amelogenin, enamelin, and enamel-processing proteases, Mmp-20 and Klk4 was reduced in the Bmp2 cKO teeth as reflected in a reduced enamel formation. Exogenous Bmp2 up-regulated those gene expressions in mouse enamel organ epithelial cells. This result for the first time indicates Bmp2 signaling is essential for proper enamel development and mineralization in vivo. J. Cell. Physiol. 230: 1871-1882, 2015.
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U2 - 10.1002/jcp.24915
DO - 10.1002/jcp.24915
M3 - Article
C2 - 25545831
AN - SCOPUS:84928389900
SN - 0021-9541
VL - 230
SP - 1871
EP - 1882
JO - Journal of Cellular Physiology
JF - Journal of Cellular Physiology
IS - 8
ER -