Bmp2 deletion causes an amelogenesis imperfecta phenotype via regulating enamel gene expression

Feng Guo, Junsheng Feng, Feng Wang, Wentong Li, Qingping Gao, Zhuo Chen, Lisa Shoff, Kevin J Donly, Jelica Gluhak-Heinrich, Yong-hee P Chun, Stephen E Harris, Mary Macdougall, Shuo Chen

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Abstract

Although Bmp2 is essential for tooth formation, the role of Bmp2 during enamel formation remains unknown in vivo. In this study, the role of Bmp2 in regulation of enamel formation was investigated by the Bmp2 conditional knock out (Bmp2 cKO) mice. Teeth of Bmp2 cKO mice displayed severe and profound phenotypes with asymmetric and misshaped incisors as well as abrasion of incisors and molars. Scanning electron microscopy analysis showed that the enamel layer was hypoplastic and enamel lacked a typical prismatic pattern. Teeth from null mice were much more brittle as tested by shear and compressive moduli. Expression of enamel matrix protein genes, amelogenin, enamelin, and enamel-processing proteases, Mmp-20 and Klk4 was reduced in the Bmp2 cKO teeth as reflected in a reduced enamel formation. Exogenous Bmp2 up-regulated those gene expressions in mouse enamel organ epithelial cells. This result for the first time indicates Bmp2 signaling is essential for proper enamel development and mineralization in vivo. J. Cell. Physiol. 230: 1871-1882, 2015.

Original languageEnglish (US)
Pages (from-to)1871-1882
Number of pages12
JournalJournal of Cellular Physiology
Volume230
Issue number8
DOIs
StatePublished - Aug 1 2015

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ASJC Scopus subject areas

  • Clinical Biochemistry
  • Cell Biology
  • Physiology

Cite this

Guo, F., Feng, J., Wang, F., Li, W., Gao, Q., Chen, Z., Shoff, L., Donly, K. J., Gluhak-Heinrich, J., Chun, Y. P., Harris, S. E., Macdougall, M., & Chen, S. (2015). Bmp2 deletion causes an amelogenesis imperfecta phenotype via regulating enamel gene expression. Journal of Cellular Physiology, 230(8), 1871-1882. https://doi.org/10.1002/jcp.24915