Blood-sucking lice may disseminate Trypanosoma cruzi infection in baboons

Enrique R. Argañaraz, Gene B. Hubbard, Larissa A. Ramos, Allen L. Ford, Nadjar Nitz, Michelle M. Leland, John L. Vandeberg, Antonio R.L. Teixeira

Research output: Contribution to journalArticlepeer-review

23 Scopus citations

Abstract

Trypanosoma cruzi (Schyzotrypanum, Chagas, 1909), and Chagas disease are endemic in captive-reared baboons at the Southwest Foundation for Biomedical Research, San Antonio, Texas. We obtained PCR amplification products from DNA extracted from sucking lice collected from the hair and skin of T. cruzi-infected baboons, with specific nested sets of primers for the protozoan kinetoplast DNA, and nuclear DNA. These products were hybridized to their complementary internal sequences. Selected sequences were cloned and sequencing established the presence of T. cruzi nuclear DNA, and minicircle kDNA. Competitive PCR with a kDNA set of primers determined the quantity of approximately 23.9 ± 18.2 T. cruzi per louse. This finding suggests that the louse may be a vector incidentally contributing to the dissemination of T. cruzi infection in the baboon colony.

Original languageEnglish (US)
Pages (from-to)271-276
Number of pages6
JournalRevista do Instituto de Medicina Tropical de Sao Paulo
Volume43
Issue number5
DOIs
StatePublished - 2001

Keywords

  • Baboons
  • Lice
  • Papio hamadrias
  • Pedicinus obtusus
  • Trypanosoma cruzi infection

ASJC Scopus subject areas

  • Infectious Diseases

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