Blockage of hemichannels alters gene expression in osteocytes in a high magneto-gravitational environment

Huiyun Xu, Dandan Ning, Dezhi Zhao, Yunhe Chen, Dongdong Zhao, Sumin Gu, Jean X. Jiang, Peng Shang

Research output: Contribution to journalArticlepeer-review

Abstract

Osteocytes, the most abundant cells in bone, are highly responsive to external environmental changes. We tested how Cx43 hemichannels which mediate the exchange of small molecules between cells and extracellular environment impact genome wide gene expression under conditions of abnormal gravity and magnetic field. To this end, we subjected osteocytic MLO-Y4 cells to a high magneto-gravitational environment and used microarray to examine global gene expression and a specific blocking antibody was used to assess the role of Cx43 hemichannels. While 3 hr exposure to abnormal gravity and magnetic field had relatively minor effects on global gene expression, blocking hemichannels significantly impacted the expression of a number of genes which are involved in cell viability, apoptosis, mineral absorption, protein absorption and digestion, and focal adhesion. Also, blocking of hemichannels enriched genes in multiple signaling pathways which are enaged by TGF-beta, Jak-STAT and VEGF. These results show the role of connexin hemichannels in bone cells in high magnetogravitational environments.

Original languageEnglish (US)
Pages (from-to)783-794
Number of pages12
JournalFrontiers in Bioscience - Landmark
Volume22
Issue number5
DOIs
StatePublished - Jan 1 2017

Keywords

  • Connexin 43
  • Gene expression profile
  • HMGE
  • Hemichannel
  • MLO-Y4 cells

ASJC Scopus subject areas

  • Biochemistry, Genetics and Molecular Biology(all)
  • Immunology and Microbiology(all)

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