Blockade by lnterleukin-1-alpha of nitricoxidergic control of luteinizing hormone-releasing hormone release in vivo and in vitro

Valeria Rettori, Nina Belova, Amrita Kamat, Krzysztof Lyson, Martha Gimeno, Samuel M. McCann

Research output: Contribution to journalArticlepeer-review

51 Scopus citations

Abstract

Nitric oxide (NO) synthase (NOS), the enzyme that converts arginine into citrullinc plus NO. the latter a highly active free radical, occurs in a large number of neurons in the brain, including certain neurons in the hypothalamus. Our previous experiments have shown that norepinephrine (NE)-induced prostaglandin E2 (PGE2) release from medial basal hypothalamic explants (MBH) is mediated by NO. Bccause release of luteinizing hormone (LH)-releasing hormone (LHRH) is also driven by NE and PGEi, we hypothesized that NO controls pulsatile release of LHRH in vivo, which in turn induces pulsatile LH release. Indeed, in vivo and in vitro experiments using an inhibitor of NOS (NG-monomethyl-L-arginine; NMMA) demonstrated that pulsatile LH release is mediated by NO; LHRH release in vitro is also mediated by this free radical. Cytokines that are released from cells of the immune system during infection also inhibit LHRH release. We compared the action of one such cytokine, interleukin-lα (IL-lα), on LHRH release with that of substances which inhibit or induce NO release. Microinjection of IL-lα (0.06 pmol in 2 μl) into the third cerebral ventricle (3V) of conscious, castrated male rats had an action similar to that of 3V microinjection of NMMA (1 mg in 5 μl): it blocked pulsatile LH, but not follicle-stimulating hormone (FSH) release. The only difference between the responses to NMMA and IL-la was that the latency to onset was greater with IL-lα. When both NMMA and IL-lα were microinjected together, there was an additive suppressive effect on LH release during the first hour after injection. As in the case of NMMA, there was no effect on pulsatile FSH release of IL-lα injected alone or together with NMMA. The latter results suggest that hypothalamic control of FSH release is distinct from that of LH and does not involve NO. Previous in vitro experiments showed that IL-la also has an action identical to that of NMMA; inhibition of NE-induced PGEi and LHRH release from MBH explants. Therefore, IL-lα appears to act on its receptors on the NOergic neurons to prevent the release of NO, or on the LHRH terminals to inhibit the response to NO. To lest the hypothesis that IL-la directly inhibits the response of LHRH terminals to NO, we incubated MBH explants with sodium nitroprusside (NP; 500 μM), which spontaneously releases NO. NP-induccd LHRH release was inhibited by IL-la (10 pM). We conclude that IL-lα directly suppresses the response of the LHRH terminals to NO.

Original languageEnglish (US)
Pages (from-to)86-91
Number of pages6
JournalNeuroImmunoModulation
Volume1
Issue number1
DOIs
StatePublished - 1994
Externally publishedYes

Keywords

  • Hemoglobin
  • N-monomethyl-L-arginine
  • Nitric oxide synthase
  • Plasma luteinizing hormone
  • Sodium nitroprusside

ASJC Scopus subject areas

  • Immunology
  • Endocrinology
  • Neurology
  • Endocrine and Autonomic Systems

Fingerprint Dive into the research topics of 'Blockade by lnterleukin-1-alpha of nitricoxidergic control of luteinizing hormone-releasing hormone release in vivo and in vitro'. Together they form a unique fingerprint.

Cite this