Block of granulocytic differentiation of 32Dcl3 cells by AML1/ETO(MTG8) but not by highly expressed Bcl-2

Hidetsugu Kohzaki, Kosei Ito, Gang Huang, Hee Jun Wee, Yota Murakami, Yoshiaki Ito

Research output: Contribution to journalArticlepeer-review

36 Scopus citations

Abstract

The chimeric gene, AML1/ETO (MTG8), generated in t(8;21) acute myeloid leukemia enhances the expression of Bcl-2. To evaluate whether this enhancement is the primary role of AML1/ETO in leukemogenesis, effects of over-expression of Bcl-2 in the murine myeloid precursor cell line, 32Dcl3, were examined. When 32Dcl3 cells expressing exogenous Bcl-2 were induced to differentiate, the onset of morphological differentiation was delayed. However, even the cells expressing very high levels of exogenous Bcl-2 eventually underwent differentiation without a significant decrease in the synthesis of Bcl-2. On the contrary, 32Dcl3 cells stably expressing AML1/ETO were completely resistant to differentiation and continued to grow in the presence of G-CSF. These results are consistent with the interpretation that stimulation of Bcl-2 expression is not the primary target of AML1/ETO.

Original languageEnglish (US)
Pages (from-to)4055-4062
Number of pages8
JournalOncogene
Volume18
Issue number28
DOIs
StatePublished - Jul 15 1999
Externally publishedYes

Keywords

  • 32Dcl3
  • AML1
  • AML1/ETO(MTG8)
  • Bcl-2
  • Granulocytic differentiation

ASJC Scopus subject areas

  • Molecular Biology
  • Genetics
  • Cancer Research

Fingerprint

Dive into the research topics of 'Block of granulocytic differentiation of 32Dcl3 cells by AML1/ETO(MTG8) but not by highly expressed Bcl-2'. Together they form a unique fingerprint.

Cite this