Bis(1,8-anilinonaphthalenesulfonate). A novel and potent inhibitor of microtubule assembly

P. Horowitz, V. Prasad, R. F. Luduena

Research output: Contribution to journalArticle

68 Citations (Scopus)

Abstract

Two related compounds, 1,8-anilinonaphthalenesulfonate (1,8-ANS) and bis(1,8-anilinonaphthalenesulfonate) (Bis-ANS), are useful fluorescent probes for hydrophobic areas on protein molecules. Using fluorescence, we examined the binding of these compounds to bovine brain tubulin and found that Bis-ANS and 1,8-ANS bound to tubulin with K(i) values of 2 and 25 μM, respectively. Bis-ANS potently inhibited the polymerization of tubulin into microtubules in vitro. In the presence of microtubule-associated protein 2, half-maximal inhibition of assembly was obtained at 3 μM Bis-ANS. In the presence of tau protein, half-maximal inhibition was obtained at 15 μM Bis-ANS. Surprisingly, 1,8-ANS, even at 200 μM, did not inhibit assembly. Scatchard analysis indicated one binding site for Bis-ANS on tubulin. Previously reports of 1,8-ANS binding to tubulin may have been influenced by the presence of Bis-ANS which until recently was a common contaminant of commercial supplies. Because of its intense fluorescence in addition to its potent inhibitory effects, Bis-ANS appears to be a useful probe to study microtubule assembly and other interactions involving tubulin.

Original languageEnglish (US)
Pages (from-to)14647-14650
Number of pages4
JournalJournal of Biological Chemistry
Volume259
Issue number23
StatePublished - 1984

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Microtubules
Tubulin
Fluorescence
tau Proteins
Microtubule-Associated Proteins
5,5'-bis(8-(phenylamino)-1-naphthalenesulfonate)
1-anilino-8-naphthalenesulfonate
Fluorescent Dyes
Polymerization
Brain
Binding Sites
Impurities
Molecules
Proteins

ASJC Scopus subject areas

  • Biochemistry

Cite this

Bis(1,8-anilinonaphthalenesulfonate). A novel and potent inhibitor of microtubule assembly. / Horowitz, P.; Prasad, V.; Luduena, R. F.

In: Journal of Biological Chemistry, Vol. 259, No. 23, 1984, p. 14647-14650.

Research output: Contribution to journalArticle

Horowitz, P, Prasad, V & Luduena, RF 1984, 'Bis(1,8-anilinonaphthalenesulfonate). A novel and potent inhibitor of microtubule assembly', Journal of Biological Chemistry, vol. 259, no. 23, pp. 14647-14650.
Horowitz, P. ; Prasad, V. ; Luduena, R. F. / Bis(1,8-anilinonaphthalenesulfonate). A novel and potent inhibitor of microtubule assembly. In: Journal of Biological Chemistry. 1984 ; Vol. 259, No. 23. pp. 14647-14650.
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