Bipartite stimulatory action of the Hop2-Mnd1 complex on the Rad51 recombinase

Peter Chi, Joseph San Filippo, Michael G. Sehorn, Galina V. Petukhova, Patrick Sung

Research output: Contribution to journalArticlepeer-review

75 Scopus citations

Abstract

The HOP2 and MND1 genes are indispensable for meiotic recombination. The products of these genes associate to form a stable heterodimeric complex that binds DNA and stimulates the recombinase activity of Rad51 and Dmc1. Here we conduct molecular studies to delineate the action mechanism of the Hop2-Mnd1 complex. We present evidence to implicate Hop2 as the major DNA-binding subunit and Mnd1 as the prominent Rad51 interaction entity. Hop2-Mnd1 stabilizes the Rad51-single-stranded DNA (ssDNA) nucleoprotein filament, the catalytic intermediate in recombination reactions. We also show that Hop2-Mnd1 enhances the ability of the Rad51-ssDNA nucleoprotein filament to capture duplex DNA, an obligatory step in the formation of the synaptic complex critical for DNA joint formation. Thus, our results unveil a bipartite mechanism of Hop2-Mnd1 in homologous DNA pairing: stabilization of the Rad51 presynaptic filament and duplex DNA capture to enhance synaptic complex formation.

Original languageEnglish (US)
Pages (from-to)1747-1757
Number of pages11
JournalGenes and Development
Volume21
Issue number14
DOIs
StatePublished - Jul 15 2007

Keywords

  • DNA repair
  • Homologous recombination
  • Rad51 recombinase
  • Synaptic complex

ASJC Scopus subject areas

  • Genetics
  • Developmental Biology

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