Biotinylated nucleic acid hybridization probes for potato virus detection

H. E. Hopp, L. Giavedoni, M. A. Mandel, A. Arese, B. Orman, F. Bravo Almonacid, H. N. Torres, A. N. Mentaberry

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    Abstract

    cDNA libraries, representative of potato viruses X (PVXc strain) and Y (PVYo strain) genomes were obtained. A PVX cDNA cloned fragment was sequenced and biotinylated to be used as hybridization probe for the detection of purified virus or nucleic acid extracts of infected plants. Dot hybridization assay was sensitive to detect 4 ng of viral particles, corresponding to about 200 pg of viral RNA. The level of detection in infected plant extracts was as effective as that obtained with the ELISA. The presence of biotinylated PVY cDNA in the hybridization mixture did not affect sensitivity of the PVX detection assay, suggesting that a single diagnostic assay for several potato viruses and virus-related pathogens could be developed.

    Original languageEnglish (US)
    Pages (from-to)231-241
    Number of pages11
    JournalArchives of Virology
    Volume103
    Issue number3-4
    DOIs
    Publication statusPublished - Sep 1 1988

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    ASJC Scopus subject areas

    • Virology

    Cite this

    Hopp, H. E., Giavedoni, L., Mandel, M. A., Arese, A., Orman, B., Almonacid, F. B., ... Mentaberry, A. N. (1988). Biotinylated nucleic acid hybridization probes for potato virus detection. Archives of Virology, 103(3-4), 231-241. https://doi.org/10.1007/BF01311095