Biotin Uptake by Basolateral Membrane Vesicles of Human Placenta: Normal Characteristics and Role of Ethanol

Zhi Qiang hu, George I. Henderson, Donald M. Mock, Steven Schenker

Research output: Contribution to journalArticlepeer-review

19 Scopus citations

Abstract

This study assessed the mechanism of uptake of biotin by the fetal-facing (basolateral) membrane of the term human placenta. Using membrane vesicles, we showed that most of the uptake was attributable to transfer of the vitamin into the vesicle and that the uptake was saturable, Na-dependent, carrier-mediated, and electroneutral. The rate of uptake was less than for biotin uptake by the maternal-facing (apical) membrane of the human placenta. Because ethanol inhibits biotin uptake by the apical membrane, the effect of ethanol on uptake by basolateral vesicles was investigated. With 10-hr exposure at a concentration of 2 and 3 mg/ml, but not 1 mg/ml, ethanol modestly inhibited biotin uptake. The mechanism of inhibition by alcohol is not known.

Original languageEnglish (US)
Pages (from-to)404-408
Number of pages5
JournalProceedings of the Society for Experimental Biology and Medicine
Volume206
Issue number4
DOIs
StatePublished - Sep 1994
Externally publishedYes

ASJC Scopus subject areas

  • Biochemistry, Genetics and Molecular Biology(all)

Fingerprint

Dive into the research topics of 'Biotin Uptake by Basolateral Membrane Vesicles of Human Placenta: Normal Characteristics and Role of Ethanol'. Together they form a unique fingerprint.

Cite this