Biotin-labeled hairpin oligonucleotides: Probes to detect double-strand breaks in DNA in apoptotic cells

Vladimir V. Didenko, James R. Tunstead, Peter J. Hornsby

Research output: Contribution to journalArticlepeer-review

58 Scopus citations

Abstract

Hairpin oligonucleotides were synthesized with stems ending in a double- stranded structure, which can be ligated to double-strand breaks in DNA, and with loops that contain nucleotides modified by the attachment of biotin. These probes specifically and sensitively detect double-strand breaks in apoptotic cells. Localization of these probes is restricted to areas of chromatin characteristic of apoptosis, whereas much more diffuse labeling was obtained when all available 3' DNA ends were labeled by terminal transferase. In principle, hairpin oligonucleotide probes can be designed with any type of 3' or 5' overhang complementary to double-strand DNA termini being detected.

Original languageEnglish (US)
Pages (from-to)897-902
Number of pages6
JournalAmerican Journal of Pathology
Volume152
Issue number4
StatePublished - Apr 1998
Externally publishedYes

ASJC Scopus subject areas

  • Pathology and Forensic Medicine

Fingerprint Dive into the research topics of 'Biotin-labeled hairpin oligonucleotides: Probes to detect double-strand breaks in DNA in apoptotic cells'. Together they form a unique fingerprint.

Cite this