TY - JOUR
T1 - Biotin-labeled hairpin oligonucleotides
T2 - Probes to detect double-strand breaks in DNA in apoptotic cells
AU - Didenko, Vladimir V.
AU - Tunstead, James R.
AU - Hornsby, Peter J.
N1 - Copyright:
Copyright 2007 Elsevier B.V., All rights reserved.
PY - 1998/4
Y1 - 1998/4
N2 - Hairpin oligonucleotides were synthesized with stems ending in a double- stranded structure, which can be ligated to double-strand breaks in DNA, and with loops that contain nucleotides modified by the attachment of biotin. These probes specifically and sensitively detect double-strand breaks in apoptotic cells. Localization of these probes is restricted to areas of chromatin characteristic of apoptosis, whereas much more diffuse labeling was obtained when all available 3' DNA ends were labeled by terminal transferase. In principle, hairpin oligonucleotide probes can be designed with any type of 3' or 5' overhang complementary to double-strand DNA termini being detected.
AB - Hairpin oligonucleotides were synthesized with stems ending in a double- stranded structure, which can be ligated to double-strand breaks in DNA, and with loops that contain nucleotides modified by the attachment of biotin. These probes specifically and sensitively detect double-strand breaks in apoptotic cells. Localization of these probes is restricted to areas of chromatin characteristic of apoptosis, whereas much more diffuse labeling was obtained when all available 3' DNA ends were labeled by terminal transferase. In principle, hairpin oligonucleotide probes can be designed with any type of 3' or 5' overhang complementary to double-strand DNA termini being detected.
UR - http://www.scopus.com/inward/record.url?scp=0031922060&partnerID=8YFLogxK
UR - http://www.scopus.com/inward/citedby.url?scp=0031922060&partnerID=8YFLogxK
M3 - Article
C2 - 9546350
AN - SCOPUS:0031922060
SN - 0002-9440
VL - 152
SP - 897
EP - 902
JO - American Journal of Pathology
JF - American Journal of Pathology
IS - 4
ER -